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Literature summary extracted from

  • Zhou, X.; Zhang, N.; Liu, L.; Walters, K.J.; Hanna, P.E.; Wagner, C.R.
    Probing the catalytic potential of the hamster arylamine N-acetyltransferase 2 catalytic triad by site-directed mutagenesis of the proximal conserved residue, Tyr190 (2009), FEBS J., 276, 6928-6941.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.5 Y190A no significant alteration in the overall fold of the protein Mesocricetus auratus
2.3.1.5 Y190F no significant alteration in the overall fold of the protein Mesocricetus auratus
2.3.1.5 Y190I no significant alteration in the overall fold of the protein Mesocricetus auratus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.5 2.8
-
 4-nitrophenyl acetate mutant Y190A, pH 7.0, 25°C Mesocricetus auratus
2.3.1.5 3.5
-
 4-nitrophenyl acetate mutant Y190F, pH 7.0, 25°C Mesocricetus auratus
2.3.1.5 4.1
-
 4-nitrophenyl acetate mutant Y190I, pH 7.0, 25°C Mesocricetus auratus
2.3.1.5 9
-
 4-nitrophenyl acetate wild-type, pH 7.0, 25°C Mesocricetus auratus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.5 Mesocricetus auratus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.5 acetyl-CoA + an arylamine = CoA + an N-acetylarylamine residue Y190 is critical for maximizing the acetylation rate of NAT2 and the transacetylation rate of substrate p-aminobenzoic acid. Y190 also plays an important role in determining the pKa of the active site Cys during acetylation, as well as the pH versus the rate profile for transacetylation Mesocricetus auratus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.5 4-nitrophenyl acetate + 4-aminobenzoic acid
-
 Mesocricetus auratus 4-nitrophenol + N-acetyl-4-aminobenzoic acid
-
 ?
2.3.1.5 4-nitrophenyl acetate + anisidine
-
 Mesocricetus auratus 4-nitrophenol + N-acetyl-anisidine
-
 ?
2.3.1.5 4-nitrophenyl acetate + N-(4-aminobenzoyl)-L-glutamate
-
 Mesocricetus auratus 4-nitrophenol + N-(4-acetylaminobenzoyl)-L-glutamate
-
 ?
2.3.1.5 acetyl-CoA + 4-aminobenzoic acid
-
 Mesocricetus auratus CoA + N-acetyl-4-aminobenzoic acid
-
 ?
2.3.1.5 acetyl-CoA + 4-nitroaniline
-
 Mesocricetus auratus CoA + N-acetyl-4-nitroaniline
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.5 0.25
-
 4-nitroaniline mutant Y190A, pH 7.0, 25°C Mesocricetus auratus
2.3.1.5 0.31
-
 4-nitroaniline mutant Y190F, pH 7.0, 25°C Mesocricetus auratus
2.3.1.5 0.6
-
 4-nitroaniline wild-type, pH 7.0, 25°C Mesocricetus auratus
2.3.1.5 0.89
-
 4-nitroaniline mutant Y190I, pH 7.0, 25°C Mesocricetus auratus
2.3.1.5 2 8 anisidine mutant Y190A, pH 7.0, 25°C Mesocricetus auratus
2.3.1.5 8
-
 4-Aminobenzoic acid cosubstrate acetyl-CoA, mutant Y190A, pH 7.0, 25°C Mesocricetus auratus
2.3.1.5 15
-
 4-nitrophenyl acetate mutant Y190A, pH 7.0, 25°C Mesocricetus auratus
2.3.1.5 16
-
 4-aminobenzoyl glutamic acid mutant Y190A, pH 7.0, 25°C Mesocricetus auratus
2.3.1.5 38
-
 4-Aminobenzoic acid cosubstrate 4-nitrophenyl acetate, mutant Y190A, pH 7.0, 25°C Mesocricetus auratus
2.3.1.5 57
-
 4-nitrophenyl acetate mutant Y190I, pH 7.0, 25°C Mesocricetus auratus
2.3.1.5 58
-
 4-Aminobenzoic acid cosubstrate acetyl-CoA, mutant Y190I, pH 7.0, 25°C Mesocricetus auratus
2.3.1.5 60
-
 4-Aminobenzoic acid cosubstrate 4-nitrophenyl acetate, mutant Y190I, pH 7.0, 25°C Mesocricetus auratus
2.3.1.5 77
-
 4-aminobenzoyl glutamic acid mutant Y190I, pH 7.0, 25°C Mesocricetus auratus
2.3.1.5 85
-
 anisidine mutant Y190I, pH 7.0, 25°C Mesocricetus auratus
2.3.1.5 86
-
 4-aminobenzoyl glutamic acid mutant Y190F, pH 7.0, 25°C Mesocricetus auratus
2.3.1.5 120
-
 4-aminobenzoyl glutamic acid wild-type, pH 7.0, 25°C Mesocricetus auratus
2.3.1.5 189
-
 4-Aminobenzoic acid cosubstrate acetyl-CoA, mutant Y190F, pH 7.0, 25°C Mesocricetus auratus
2.3.1.5 200
-
 4-Aminobenzoic acid cosubstrate acetyl-CoA, wild-type, pH 7.0, 25°C Mesocricetus auratus
2.3.1.5 260
-
 anisidine wild-type, pH 7.0, 25°C Mesocricetus auratus
2.3.1.5 279
-
 4-nitrophenyl acetate mutant Y190F, pH 7.0, 25°C Mesocricetus auratus
2.3.1.5 288
-
 anisidine mutant Y190F, pH 7.0, 25°C Mesocricetus auratus
2.3.1.5 393
-
 4-Aminobenzoic acid cosubstrate 4-nitrophenyl acetate, mutant Y190F, pH 7.0, 25°C Mesocricetus auratus
2.3.1.5 620
-
 4-Aminobenzoic acid cosubstrate 4-nitrophenyl acetate, wild-type, pH 7.0, 25°C Mesocricetus auratus
2.3.1.5 1301
-
 4-nitrophenyl acetate wild-type, pH 7.0, 25°C Mesocricetus auratus