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Literature summary extracted from

  • Storbeck, S.; Walther, J.; Mueller, J.; Parmar, V.; Schiebel, H.M.; Kemken, D.; Duelcks, T.; Warren, M.J.; Layer, G.
    The Pseudomonas aeruginosa nirE gene encodes the S-adenosyl-L-methionine-dependent uroporphyrinogen III methyltransferase required for heme d1 biosynthesis (2009), FEBS J., 276, 5973-5982.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.107 recombinant expression of C-terminally His-tagged NirE in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH10B Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.107 additional information construction of a nirE transposon mutant, which is not complemented by native cobA encoding the SAM-dependent uroporphyrinogen III methyltransferase involved in cobalamin formation, overview Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.107 S-adenosyl-L-homocysteine product inhibition at concentrations above 0.002 mM Pseudomonas aeruginosa
2.1.1.107 uroporphyrinogen III substrate inhibition at concentrations above 0.017 mM Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.107 30000
-
 2 * 30000, SDS-PAGE Pseudomonas aeruginosa
2.1.1.107 60000
-
 gel filtration Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.107 uroporphyrinogen III + S-adenosyl-L-methionine Pseudomonas aeruginosa
-
 precorrin-2 + S-adenosyl-L-homocysteine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.107 Pseudomonas aeruginosa
-
 gene nirE
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.107 recombinant His-tagged NirE from Escherichia coli by metal chelate chromatography and gel filtration to homogeneity Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.107 0.000044
-
 recombinant enzyme Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.107 additional information at high enzyme concentrations NirE catalyzes an overmethylation of uroporphyrinogen III, resulting in the formation of trimethylpyrrocorphin Pseudomonas aeruginosa ?
-
 ?
2.1.1.107 uroporphyrinogen III + S-adenosyl-L-methionine
-
 Pseudomonas aeruginosa precorrin-2 + S-adenosyl-L-homocysteine
-
 ?
2.1.1.107 uroporphyrinogen III + S-adenosyl-L-methionine NirE binds S-adenosyl-L-methionine, although not with the same avidity as reported for other SAM-dependent uroporphyrinogen III methyltransferases involved in siroheme and cobalamin biosynthesis Pseudomonas aeruginosa precorrin-2 + S-adenosyl-L-homocysteine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.107 homodimer 2 * 30000, SDS-PAGE Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
2.1.1.107 NirE
-
 Pseudomonas aeruginosa
2.1.1.107 SAM-dependent uroporphyrinogen III methyltransferase
-
 Pseudomonas aeruginosa
2.1.1.107 SUMT
-
 Pseudomonas aeruginosa
2.1.1.107 uroporphyrinogen III methyltransferase
-
 Pseudomonas aeruginosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.107 37
-
 assay at Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.107 8
-
 assay at Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.107 S-adenosyl-L-methionine dependent on Pseudomonas aeruginosa

General Information

EC Number General Information Comment Organism
2.1.1.107 metabolism the enzyme is important in the heme d1 biosynthesis, the essential prosthetic group of the dissimilatory nitrite reductase cytochrome cd1 requiring the methylation of the tetrapyrrole precursor uroporphyrinogen III at positions C-2 and C-7, pathway overview Pseudomonas aeruginosa