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Literature summary extracted from

  • Wang, J.; Harris, J.; Mousseau, D.D.; Edmondson, D.E.
    Mutagenic probes of the role of Ser209 on the cavity shaping loop of human monoamine oxidase A (2009), FEBS J., 276, 4569-4581.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
1.4.3.4 S209A site-directed mutagenesis of the phosphorylation site. The MAO A S209A mutant exhibits similar catalytic properties to those of wild-type enzyme, its catalytic activity is reduced by 1.5-3.7fold compared to the wild-type MAO A Homo sapiens
1.4.3.4 S209E site-directed mutagenesis of the phosphorylation site. Compared with purified wild-type and S209A MAO A proteins, the S209G MAO A mutant shows 10fold reduced kcat values and 10fold increased Km values compared to the wild-type enzymesignificant differences in covalent flavin fluorescence yield, circular dichroism spectra and thermal stability Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.4 Clorgyline an acetylenic inhibitor, addition of it results in the conversion of the oxidized flavin cofactors to their respective N(5) flavocyanine adducts Homo sapiens
1.4.3.4 D-amphetamine a nonselective reversible MAO inhibitor, inhibits the MAO A S209E mutant with much lower affinity, 160fold and 20fold, respectively, than the wild-type enzyme Homo sapiens
1.4.3.4 Harmane an MAO A specific reversible inhibitor Homo sapiens
1.4.3.4 isatin a nonselective reversible MAO inhibitor, inhibits the MAO A S209E mutant with much lower affinity, 160fold and 20fold, respectively, than the wild-type enzyme Homo sapiens
1.4.3.4 phentermine binding to the S209E mutant is reduced by 13fold compatred to the wild-type MAO A Homo sapiens
1.4.3.4 pirlindole an MAO A specific reversible inhibitor Homo sapiens
1.4.3.4 tetrindole an MAO A specific reversible inhibitor Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.4.3.4 mitochondrial outer membrane
-
 Homo sapiens 5741
-

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.4 Homo sapiens P21397
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.4.3.4 flavoprotein
-
 Homo sapiens
1.4.3.4 phosphoprotein MAO A is phosphorylated at Ser209, which does not play a role in enzyme regulation, overview Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.4 benzylamine + H2O + O2 MAO-A Homo sapiens benzaldehyde + NH3 + H2O2
-
 ?
1.4.3.4 kynuramine + H2O + O2 MAO-A Homo sapiens 3-(2-aminophenyl)-3-oxopropanal + NH3 + H2O2
-
 ?
1.4.3.4 additional information structure-activity studies on wild-type and mutant enzymes, active site structure, overview Homo sapiens ?
-
 ?
1.4.3.4 phenylethylamine + H2O + O2 MAO-A Homo sapiens 2-phenylethanal + NH3 + H2O2
-
 ?
1.4.3.4 serotonin + H2O + O2 MAO-A Homo sapiens (5-hydroxy-1H-indol-3-yl)acetaldehyde + NH3 + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.4 More structure-activity studies on wild-type and mutant enzymes, active site structure, overview Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.4.3.4 MAO A
-
 Homo sapiens
1.4.3.4 monoamine oxidase A
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.4 flavin comparison of flavin fluorescence and circular dichroism spectral properties of wild-type MAO A and of the MAO A Ser209 mutant proteins Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.4.3.4 6.682
-
 phentermine
-
 Homo sapiens