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Literature summary extracted from

  • Hrmova, M.; Farkas, V.; Harvey, A.J.; Lahnstein, J.; Wischmann, B.; Kaewthai, N.; Ezcurra, I.; Teeri, T.T.; Fincher, G.B.
    Substrate specificity and catalytic mechanism of a xyloglucan xyloglucosyl transferase HvXET6 from barley (Hordeum vulgare L.) (2009), FEBS J., 276, 437-456.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.207 expressed in Pichia pastoris Hordeum vulgare

General Stability

EC Number General Stability Organism
2.4.1.207 repeated cycles of freezing and thawing reduce its activity to around 10% Hordeum vulgare

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.207 0.0085
-
 reduced xyloglucan-derived heptasaccharide recombinant isozyme XET6, at 30°C in 100 mM sodium succinate buffer, pH 6.0, containing 5 mM CaCl2 Hordeum vulgare
2.4.1.207 0.0399
-
 reduced xyloglucan-derived heptasaccharide purified isozyme XET5, at 30°C in 100 mM sodium succinate buffer, pH 6.0, containing 5 mM CaCl2 Hordeum vulgare
2.4.1.207 1.6
-
 donor Glc8-based xyloglucan oligosaccharide recombinant isozyme XET6, at 30°C in 100 mM sodium succinate buffer, pH 6.0, containing 5 mM CaCl2 Hordeum vulgare

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.207 33000 35000 SDS-PAGE shows a single, but somewhat diffuse band of 33-35 kDa, indicating that the protein could be glycosylated Hordeum vulgare

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.207 Hordeum vulgare B1P1S7 isozyme XET6
-
2.4.1.207 Hordeum vulgare P93668
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.207 ammonium sulfate precipitation, Sepharose Q column chromatography, phenyl Sepharose column chromatography and Bio-Gel P60 gel filtration Hordeum vulgare

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.207 seedling
-
 Hordeum vulgare
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.207 0.072
-
 enzyme from crude homogenate Hordeum vulgare
2.4.1.207 31.07
-
 after 432fold purification Hordeum vulgare

Storage Stability

EC Number Storage Stability Organism
2.4.1.207 -20°C or -80°C, activity declines gradually Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.207 donor Glc8-based xyloglucan oligosaccharide + acceptor sulforhodamine-labelled xyloglucan oligosaccharide
-
 Hordeum vulgare ?
-
 ?
2.4.1.207 Glc8-based xyloglucan oligosaccharide + ?
-
 Hordeum vulgare ?
-
 ?
2.4.1.207 hydroxyethyl cellulose + reduced xyloglucan-derived heptasaccharide 3.4% activity compared to tamarind xyloglycan Hordeum vulgare ?
-
 ?
2.4.1.207 additional information no activity towards barley 1,3-1,4-beta-D-glucan (high viscosity), and locust bean gum galactomannan, no transferase activities are detected with laminarin, lichenin, pustulan, barley arabinoxylan, Konjac glucomannan, citrus fruit pectin and esterified pectin (potassium salt), orange polygalacturonic acid (sodium salt), soybean pectic fibre rhamnogalactouronan, beta-D-galactans (from lupin and potato) and arabinogalactan protein (from gum arabic) Hordeum vulgare ?
-
 ?
2.4.1.207 additional information no hydrolytic activity can be detected with either isoenzyme Hordeum vulgare ?
-
 ?
2.4.1.207 pea xyloglucan + reduced xyloglucan-derived heptasaccharide
-
 Hordeum vulgare ?
-
 ?
2.4.1.207 tamarind xyloglucan + reduced xyloglucan-derived heptasaccharide 100% activity Hordeum vulgare ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.207 XET
-
 Hordeum vulgare
2.4.1.207 XET5 isozyme Hordeum vulgare
2.4.1.207 XET6 isozyme Hordeum vulgare
2.4.1.207 xyloglucan endotransglycosylase
-
 Hordeum vulgare
2.4.1.207 xyloglucan xyloglucosyl transferase
-
 Hordeum vulgare

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.207 95
-
 about 25% of isozyme XET6 activity survives incubation at 95°C for 1 min Hordeum vulgare

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.207 0.0024
-
 reduced xyloglucan-derived heptasaccharide purified isozyme XET5, at 30°C in 100 mM sodium succinate buffer, pH 6.0, containing 5 mM CaCl2 Hordeum vulgare
2.4.1.207 0.0089
-
 reduced xyloglucan-derived heptasaccharide recombinant isozyme XET6, at 30°C in 100 mM sodium succinate buffer, pH 6.0, containing 5 mM CaCl2 Hordeum vulgare
2.4.1.207 0.139
-
 Glc8-based xyloglucan oligosaccharide recombinant isozyme XET6, at 30°C in 100 mM sodium succinate buffer, pH 6.0, containing 5 mM CaCl2 Hordeum vulgare

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.1.207 Hordeum vulgare isoelectric focusing
-
 6.7

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.207 0.0869
-
 Glc8-based xyloglucan oligosaccharide recombinant isozyme XET6, at 30°C in 100 mM sodium succinate buffer, pH 6.0, containing 5 mM CaCl2 Hordeum vulgare
2.4.1.207 0.2
-
 reduced xyloglucan-derived heptasaccharide purified isozyme XET5, at 30°C in 100 mM sodium succinate buffer, pH 6.0, containing 5 mM CaCl2 Hordeum vulgare
2.4.1.207 1.1
-
 reduced xyloglucan-derived heptasaccharide recombinant isozyme XET6, at 30°C in 100 mM sodium succinate buffer, pH 6.0, containing 5 mM CaCl2 Hordeum vulgare