Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Leemhuis, H.; Nightingale, K.P.; Hollfelder, F.
    Directed evolution of a histone acetyltransferase - enhancing thermostability, whilst maintaining catalytic activity and substrate specificity (2008), FEBS J., 275, 5635-5647.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.48 D551G/V582A/D639E random and site-directed mutagenesis Homo sapiens
2.3.1.48 D639V random mutagenesis Homo sapiens
2.3.1.48 E611K random mutagenesis Homo sapiens
2.3.1.48 E649D random mutagenesis Homo sapiens
2.3.1.48 F605L random mutagenesis Homo sapiens
2.3.1.48 H524Q random mutagenesis Homo sapiens
2.3.1.48 H592R random mutagenesis Homo sapiens
2.3.1.48 H600R random mutagenesis Homo sapiens
2.3.1.48 I511T/M529I random and site-directed mutagenesis Homo sapiens
2.3.1.48 K542E/D601G/Y612C random and site-directed mutagenesis Homo sapiens
2.3.1.48 K542E/D639E random and site-directed mutagenesis Homo sapiens
2.3.1.48 K627R/D639E random and site-directed mutagenesis Homo sapiens
2.3.1.48 L503P/D601G random and site-directed mutagenesis Homo sapiens
2.3.1.48 L503P/D601G/Y612C random and site-directed mutagenesis Homo sapiens
2.3.1.48 additional information development of a directed evolution protocol that allows the screening of hundreds of histone acetyltransferase mutants, generated by error-prone PCR mutagenesis, for histone acetylating activity, and to identify enhanced thermostability of the human P/CAF histone acetyltransferase. Method development, evaluation, and optimization. Directed evolution results in 24 mutants which show higher thermostability without lowering the catalytic efficiency and substrate specificity, overview. The stabilizing mutations are predominately located at surface of the enzyme Homo sapiens
2.3.1.48 N504I/Q519L/V572A/V582A random and site-directed mutagenesis Homo sapiens
2.3.1.48 P655R random mutagenesis Homo sapiens
2.3.1.48 Q519R random mutagenesis Homo sapiens
2.3.1.48 T535A/D551G random and site-directed mutagenesis Homo sapiens
2.3.1.48 V582A random mutagenesis Homo sapiens
2.3.1.48 V582A/D639E random and site-directed mutagenesis Homo sapiens
2.3.1.48 V582A/Y612C random and site-directed mutagenesis Homo sapiens
2.3.1.48 V582D random mutagenesis Homo sapiens
2.3.1.48 V582D/D639V random and site-directed mutagenesis Homo sapiens
2.3.1.48 Y612C random mutagenesis Homo sapiens
2.3.1.48 Y612C/P655R random and site-directed mutagenesis Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.48 0.00027
-
 acetyl-CoA pH 7.5, 25°C, mutants D639E and L503P/D601G/Y612C, with substrate histone H3 Homo sapiens
2.3.1.48 0.00028
-
 acetyl-CoA pH 7.5, 25°C, wild-type P/CAF enzymem, with substrate histone H3 Homo sapiens
2.3.1.48 0.00029
-
 acetyl-CoA pH 7.5, 25°C, mutant Y612C/P655R, with substrate histone H3 Homo sapiens
2.3.1.48 0.00033
-
 acetyl-CoA pH 7.5, 25°C, mutant L503P, with substrate histone H3 Homo sapiens
2.3.1.48 0.00034
-
 acetyl-CoA pH 7.5, 25°C, mutants D601G and V582A/D639E, with substrate histone H3 Homo sapiens
2.3.1.48 0.00036
-
 acetyl-CoA pH 7.5, 25°C, mutant V582A, with substrate histone H3 Homo sapiens
2.3.1.48 0.00041
-
 acetyl-CoA pH 7.5, 25°C, mutant Y612C, with substrate histone H3 Homo sapiens
2.3.1.48 0.039
-
 histone H3 pH 7.5, 25°C, mutant D639E Homo sapiens
2.3.1.48 0.053
-
 histone H3 pH 7.5, 25°C, wild-type P/CAF enzyme Homo sapiens
2.3.1.48 0.054
-
 histone H3 pH 7.5, 25°C, mutant L503P Homo sapiens
2.3.1.48 0.055
-
 histone H3 pH 7.5, 25°C, mutant D601G Homo sapiens
2.3.1.48 0.062
-
 histone H3 pH 7.5, 25°C, mutant Y612C/P655R Homo sapiens
2.3.1.48 0.064
-
 histone H3 pH 7.5, 25°C, mutant L503P/D601G/Y612C Homo sapiens
2.3.1.48 0.11
-
 histone H4 pH 7.5, 25°C, mutant L503P/D601G/Y612C Homo sapiens
2.3.1.48 0.12
-
 histone H4 pH 7.5, 25°C, mutant Y612C Homo sapiens
2.3.1.48 0.14
-
 histone H4 pH 7.5, 25°C, mutant Y612C/P655R Homo sapiens
2.3.1.48 0.15
-
 histone H4 pH 7.5, 25°C, mutant D639E Homo sapiens
2.3.1.48 0.165
-
 histone H3 pH 7.5, 25°C, mutant Y612C Homo sapiens
2.3.1.48 0.19
-
 histone H4 pH 7.5, 25°C, wild-type P/CAF enzyme Homo sapiens
2.3.1.48 0.21
-
 histone H4 pH 7.5, 25°C, mutant L503P Homo sapiens
2.3.1.48 0.25
-
 histone H4 pH 7.5, 25°C, mutant D601G Homo sapiens
2.3.1.48 0.31
-
 histone H4 pH 7.5, 25°C, mutant V582A/D639E Homo sapiens
2.3.1.48 0.46
-
 histone H3 pH 7.5, 25°C, mutant V582A/D639E Homo sapiens
2.3.1.48 0.5
-
 histone H4 pH 7.5, 25°C, mutant V582A Homo sapiens
2.3.1.48 0.56
-
 histone H3 pH 7.5, 25°C, mutant V582A Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.48 nucleus
-
 Homo sapiens 5634
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.48 acetyl-CoA + histone H3 Homo sapiens
-
 CoA + acetylhistone H3
-
 ?
2.3.1.48 acetyl-CoA + histone H4 Homo sapiens
-
 CoA + acetylhistone H4
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.48 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.48 acetyl-CoA + histone H3
-
 Homo sapiens CoA + acetylhistone H3
-
 ?
2.3.1.48 acetyl-CoA + histone H3 substrate is a H3 peptide Homo sapiens CoA + acetylhistone H3
-
 ?
2.3.1.48 acetyl-CoA + histone H4
-
 Homo sapiens CoA + acetylhistone H4
-
 ?
2.3.1.48 acetyl-CoA + histone H4 substrate is a H4 peptide Homo sapiens CoA + acetylhistone H4
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.48 p/CAF
-
 Homo sapiens
2.3.1.48 P/CAF histone acetyltransferase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.48 22 37 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.48 0.0018
-
 histone H4 pH 7.5, 25°C, mutant Y612C Homo sapiens
2.3.1.48 0.003
-
 histone H4 pH 7.5, 25°C, mutant L503P/D601G/Y612C Homo sapiens
2.3.1.48 0.0035
-
 histone H4 pH 7.5, 25°C, mutant Y612C/P655R Homo sapiens
2.3.1.48 0.0048
-
 histone H4 pH 7.5, 25°C, wild-type P/CAF enzyme and mutant V582A Homo sapiens
2.3.1.48 0.0053
-
 histone H4 pH 7.5, 25°C, mutant L503P Homo sapiens
2.3.1.48 0.0055
-
 histone H4 pH 7.5, 25°C, mutant D601G Homo sapiens
2.3.1.48 0.0058
-
 histone H4 pH 7.5, 25°C, mutant D639E Homo sapiens
2.3.1.48 0.0062
-
 histone H4 pH 7.5, 25°C, mutant V582A/D639E Homo sapiens
2.3.1.48 0.18
-
 histone H3 pH 7.5, 25°C, mutants V582A and Y612C Homo sapiens
2.3.1.48 0.2
-
 histone H3 pH 7.5, 25°C, wild-type P/CAF enzyme, and mutants L503P, D601G, D639E, Y612C/P655R, and L503P/D601G/Y612C Homo sapiens
2.3.1.48 0.22
-
 histone H3 pH 7.5, 25°C, mutant V582A/D639E Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.48 7.5
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.48 acetyl-CoA
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
2.3.1.48 physiological function histone acetylation by the enzyme plays an integral role in the epigenetic regulation of gene expression Homo sapiens