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Literature summary extracted from

  • Visser, D.F.; Hennessy, F.; Rashamuse, K.; Louw, M.E.; Brady, D.
    Cloning, purification and characterisation of a recombinant purine nucleoside phosphorylase from Bacillus halodurans Alk36 (2010), Extremophiles, 14, 185-192.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.1 expression in Escherichia coli Halalkalibacterium halodurans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.1 0.206
-
guanosine pH 7.5 Halalkalibacterium halodurans
2.4.2.1 0.236
-
Inosine pH 7.5 Halalkalibacterium halodurans

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.1 Halalkalibacterium halodurans C7FLN0
-
-
2.4.2.1 Halalkalibacterium halodurans Alk36 C7FLN0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.1 recombinant protein Halalkalibacterium halodurans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.1 30.23
-
pH 7.5, substrate inosine Halalkalibacterium halodurans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.1 guanosine + phosphate
-
Halalkalibacterium halodurans guanine + alpha-D-ribose 1-phosphate
-
?
2.4.2.1 guanosine + phosphate
-
Halalkalibacterium halodurans Alk36 guanine + alpha-D-ribose 1-phosphate
-
?
2.4.2.1 inosine + phosphate
-
Halalkalibacterium halodurans hypoxanthine + alpha-D-ribose 1-phosphate
-
?
2.4.2.1 inosine + phosphate
-
Halalkalibacterium halodurans Alk36 hypoxanthine + alpha-D-ribose 1-phosphate
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.1 70
-
-
Halalkalibacterium halodurans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.2.1 32 74 more than 60% of maximum activity Halalkalibacterium halodurans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.1 60
-
half-life 20.8 h Halalkalibacterium halodurans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.1 12
-
guanosine pH 7.5 Halalkalibacterium halodurans
2.4.2.1 28000
-
Inosine pH 7.5 Halalkalibacterium halodurans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.1 7
-
-
Halalkalibacterium halodurans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.2.1 5.7 8.4
-
Halalkalibacterium halodurans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.2.1 58.7
-
guanosine pH 7.5 Halalkalibacterium halodurans
2.4.2.1 120000
-
Inosine pH 7.5 Halalkalibacterium halodurans