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Literature summary extracted from

  • Mendoza-Macias, C.L.; Barrios-Ceballos, M.P.; Anaya-Velazquez, F.; Nakada-Tsukui, K.; Nozaki, T.; Padilla-Vaca, F.
    Entamoeba histolytica: Molecular cloning and characterization of a novel neutral sphingomyelinase (2010), Exp. Parasitol., 125, 279-285.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.12 expressed in Escherichia coli BL21 AI cells Entamoeba histolytica

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.12 additional information EDTA or EGTA in the reaction mixture have no effect on the basal activity Entamoeba histolytica
3.1.4.12 scyphostatin specific inhibitor of nSMase Entamoeba histolytica
3.1.4.12 Zn2+ nSMase activity of the recombinant protein nSM1 abolishes (12% residual activity) by 5 mM Zn2+ Entamoeba histolytica

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.12 cytosol
-
 Entamoeba histolytica 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.12 Mn2+ nSMase activity of the recombinant protein nSM1 increases 4.5fold in the presence of 0.5 mM Mn2+ Entamoeba histolytica
3.1.4.12 additional information Mg2+, Mn2+, Ca2+ and Zn2+, or Ca2+ have no significant effect on the activity Entamoeba histolytica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.12 35000
-
 isoform nSM1, SDS-PAGE Entamoeba histolytica
3.1.4.12 37000
-
 isoform nSM3, SDS-PAGE Entamoeba histolytica

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.12 Entamoeba histolytica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.12 Ni-NTA agarose column chromatography, gel filtration Entamoeba histolytica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.12 trophozoite
-
 Entamoeba histolytica
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.12 sphingomyelin + H2O
-
 Entamoeba histolytica N-acylsphingosine + choline phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.12 neutral sphingomyelinase
-
 Entamoeba histolytica
3.1.4.12 nSM1 isoform Entamoeba histolytica
3.1.4.12 nSM3 isoform Entamoeba histolytica
3.1.4.12 nSMase
-
 Entamoeba histolytica

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.1.4.12 2
-
 in 100 mM Tris-HCl buffer (pH 7.5), at 37°C Entamoeba histolytica Zn2+