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Literature summary extracted from

  • Li, Z.; Umeyama, T.; Li, Z.; Wang, C.C.
    Polo-like kinase guides cytokinesis in Trypanosoma brucei through an indirect means (2010), Eukaryot. Cell, 9, 705-716.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.21 a DNA fragment encoding the N-terminus of the TbPLK gene product is cloned into the tetracycline-inducible RNAi vector pZJM for knockdown of TbPLK expression, for tagging proteins TbPLK is cloned into the pC-EYFP-Bla vector, a plasmid for expressing glutathione S-transferase-TbPLK is constructed by cloning a DNA fragment containing full-length TbPLK into the vector pET41a Trypanosoma brucei

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.21 5-(5,6-dimethoxy-1H-benzimidazole-1-yl)-3-[2-(trifluoromethyl)-benzyloxy]-thiophene-2-carboxamide GW843682X Trypanosoma brucei
2.7.11.21 RNAi
-
 Trypanosoma brucei

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.21 cytoplasm TbPLK trans-localizes from the flagellum attachment zone to the cytoplasm during anaphase Trypanosoma brucei 5737
-
2.7.11.21 flagellum TbPLK trans-localizes from the flagellum attachment zone to the cytoplasm during anaphase Trypanosoma brucei
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.21 ATP + a protein Trypanosoma brucei
-
 ADP + a phosphoprotein
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.21 Trypanosoma brucei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.21 by glutathione-Sepharose 4B purification Trypanosoma brucei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.21 ATP + a protein
-
 Trypanosoma brucei ADP + a phosphoprotein
-
 ?
2.7.11.21 ATP + casein activity assay Trypanosoma brucei ADP + a phosphorylated casein
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.21 polo-like kinase
-
 Trypanosoma brucei
2.7.11.21 TbPLK
-
 Trypanosoma brucei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.21 37
-
 kinase assay Trypanosoma brucei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.21 7.5
-
 kinase assay Trypanosoma brucei

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.21 ATP
-
 Trypanosoma brucei

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.11.21 0.0000022
-
-
 Trypanosoma brucei 5-(5,6-dimethoxy-1H-benzimidazole-1-yl)-3-[2-(trifluoromethyl)-benzyloxy]-thiophene-2-carboxamide
2.7.11.21 0.0013
-
 Escherichia coli-expressed recombinant GST-tagged TbPLK Trypanosoma brucei 5-(5,6-dimethoxy-1H-benzimidazole-1-yl)-3-[2-(trifluoromethyl)-benzyloxy]-thiophene-2-carboxamide

General Information

EC Number General Information Comment Organism
2.7.11.21 physiological function TbPLK regulates cytokinetic initiation Trypanosoma brucei