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Literature summary extracted from

  • Xu, W.; Yan, M.; Xu, L.; Ding, L.; Ouyang, P.
    Engineering the activity of thermophilic xylose isomerase by site-directed mutation at subunit interfaces (2009), Enzyme Microb. Technol., 44, 77-83.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.5 expression of mutant enzymes in Escherichia coli strain BL21 (DE3) Thermus thermophilus

Protein Variants

EC Number Protein Variants Comment Organism
5.3.1.5 N91D site-directed mutagenesis Thermus thermophilus
5.3.1.5 N91D/D375G site-directed mutagenesis, the mutant shows increased activity but reduced thermostability compared to the wild-type enzyme Thermus thermophilus
5.3.1.5 N91D/D375G/V385A site-directed mutagenesis, the mutant shows increased activity but reduced thermostability compared to the wild-type enzyme Thermus thermophilus
5.3.1.5 N91D/K355A site-directed mutagenesis, the mutant shows increased activity but reduced thermostability compared to the wild-type enzyme Thermus thermophilus
5.3.1.5 N91D/V144A site-directed mutagenesis, the mutant shows increased activity but reduced thermostability compared to the wild-type enzyme Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.5 11.7
-
D-xylose pH 7.0, 60°C, mutant N91D/D375G Thermus thermophilus
5.3.1.5 11.8
-
D-xylose pH 7.0, 60°C, mutant N91D/K355A Thermus thermophilus
5.3.1.5 15.2
-
D-xylose pH 7.0, 60°C, mutant N91D Thermus thermophilus
5.3.1.5 21.1
-
D-xylose pH 7.0, 60°C, mutant N91D/D375G/V385A Thermus thermophilus
5.3.1.5 27.1
-
D-xylose pH 7.0, 60°C, mutant N91D/V144A Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.1.5 D-Xylose Thermus thermophilus
-
D-Xylulose
-
?

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.5 Thermus thermophilus P26997
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.5 D-Xylose
-
Thermus thermophilus D-Xylulose
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.5 60
-
assay at Thermus thermophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5.3.1.5 40 90 temperature profiles of recombinant mutant enzymes, overview Thermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.3.1.5 40 90 thermostability of recombinant mutant enzymes, overview Thermus thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.5 50.7
-
D-xylose pH 7.0, 60°C, mutant N91D Thermus thermophilus
5.3.1.5 61.65
-
D-xylose pH 7.0, 60°C, mutant N91D/D375G/V385A Thermus thermophilus
5.3.1.5 68.39
-
D-xylose pH 7.0, 60°C, mutant N91D/D375G Thermus thermophilus
5.3.1.5 116
-
D-xylose pH 7.0, 60°C, mutant N91D/K355A Thermus thermophilus
5.3.1.5 160.5
-
D-xylose pH 7.0, 60°C, mutant N91D/V144A Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.5 7
-
assay at Thermus thermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.3.1.5 5 9 pH profiles of recombinant mutant enzymes, overview Thermus thermophilus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.3.1.5 2.92
-
D-xylose pH 7.0, 60°C, mutant N91D/D375G/V385A Thermus thermophilus
5.3.1.5 3.35
-
D-xylose pH 7.0, 60°C, mutant N91D Thermus thermophilus
5.3.1.5 5.87
-
D-xylose pH 7.0, 60°C, mutant N91D/D375G Thermus thermophilus
5.3.1.5 5.92
-
D-xylose pH 7.0, 60°C, mutant N91D/V144A Thermus thermophilus
5.3.1.5 9.8
-
D-xylose pH 7.0, 60°C, mutant N91D/K355A Thermus thermophilus