EC Number | Cloned (Comment) | Organism |
---|---|---|
5.3.1.5 | expression of mutant enzymes in Escherichia coli strain BL21 (DE3) | Thermus thermophilus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
5.3.1.5 | N91D | site-directed mutagenesis | Thermus thermophilus |
5.3.1.5 | N91D/D375G | site-directed mutagenesis, the mutant shows increased activity but reduced thermostability compared to the wild-type enzyme | Thermus thermophilus |
5.3.1.5 | N91D/D375G/V385A | site-directed mutagenesis, the mutant shows increased activity but reduced thermostability compared to the wild-type enzyme | Thermus thermophilus |
5.3.1.5 | N91D/K355A | site-directed mutagenesis, the mutant shows increased activity but reduced thermostability compared to the wild-type enzyme | Thermus thermophilus |
5.3.1.5 | N91D/V144A | site-directed mutagenesis, the mutant shows increased activity but reduced thermostability compared to the wild-type enzyme | Thermus thermophilus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.1.5 | 11.7 | - |
D-xylose | pH 7.0, 60°C, mutant N91D/D375G | Thermus thermophilus | |
5.3.1.5 | 11.8 | - |
D-xylose | pH 7.0, 60°C, mutant N91D/K355A | Thermus thermophilus | |
5.3.1.5 | 15.2 | - |
D-xylose | pH 7.0, 60°C, mutant N91D | Thermus thermophilus | |
5.3.1.5 | 21.1 | - |
D-xylose | pH 7.0, 60°C, mutant N91D/D375G/V385A | Thermus thermophilus | |
5.3.1.5 | 27.1 | - |
D-xylose | pH 7.0, 60°C, mutant N91D/V144A | Thermus thermophilus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.3.1.5 | D-Xylose | Thermus thermophilus | - |
D-Xylulose | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.3.1.5 | Thermus thermophilus | P26997 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.3.1.5 | D-Xylose | - |
Thermus thermophilus | D-Xylulose | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.3.1.5 | 60 | - |
assay at | Thermus thermophilus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.3.1.5 | 40 | 90 | temperature profiles of recombinant mutant enzymes, overview | Thermus thermophilus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.3.1.5 | 40 | 90 | thermostability of recombinant mutant enzymes, overview | Thermus thermophilus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.1.5 | 50.7 | - |
D-xylose | pH 7.0, 60°C, mutant N91D | Thermus thermophilus | |
5.3.1.5 | 61.65 | - |
D-xylose | pH 7.0, 60°C, mutant N91D/D375G/V385A | Thermus thermophilus | |
5.3.1.5 | 68.39 | - |
D-xylose | pH 7.0, 60°C, mutant N91D/D375G | Thermus thermophilus | |
5.3.1.5 | 116 | - |
D-xylose | pH 7.0, 60°C, mutant N91D/K355A | Thermus thermophilus | |
5.3.1.5 | 160.5 | - |
D-xylose | pH 7.0, 60°C, mutant N91D/V144A | Thermus thermophilus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.3.1.5 | 7 | - |
assay at | Thermus thermophilus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
5.3.1.5 | 5 | 9 | pH profiles of recombinant mutant enzymes, overview | Thermus thermophilus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.1.5 | 2.92 | - |
D-xylose | pH 7.0, 60°C, mutant N91D/D375G/V385A | Thermus thermophilus | |
5.3.1.5 | 3.35 | - |
D-xylose | pH 7.0, 60°C, mutant N91D | Thermus thermophilus | |
5.3.1.5 | 5.87 | - |
D-xylose | pH 7.0, 60°C, mutant N91D/D375G | Thermus thermophilus | |
5.3.1.5 | 5.92 | - |
D-xylose | pH 7.0, 60°C, mutant N91D/V144A | Thermus thermophilus | |
5.3.1.5 | 9.8 | - |
D-xylose | pH 7.0, 60°C, mutant N91D/K355A | Thermus thermophilus |