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Literature summary extracted from

  • Saum, R.; Mingote, A.; Santos, H.; Mueller, V.
    A novel limb in the osmoregulatory network of Methanosarcina mazei Goe1: N(epsilon)-acetyl-beta-lysine can be substituted by glutamate and alanine (2009), Environ. Microbiol., 11, 1056-1065.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
5.4.3.2 DELTAabl::pac deletion mutant, loss of the ability to synthesise Nepsilon-acetyl-beta-lysine Methanosarcina mazei Go1

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.264 acetyl-CoA + (3S)-3,6-diaminohexanoate Methanosarcina mazei
-
 CoA + (3S)-6-acetamido-3-aminohexanoate
-
 ?
5.4.3.2 alpha-lysine Methanosarcina mazei Go1
-
 beta-lysine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.264 Methanosarcina mazei Q8PYC8
-
-
5.4.3.2 Methanosarcina mazei Go1
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.264 acetyl-CoA + (3S)-3,6-diaminohexanoate
-
 Methanosarcina mazei CoA + (3S)-6-acetamido-3-aminohexanoate
-
 ?
5.4.3.2 alpha-lysine
-
 Methanosarcina mazei Go1 beta-lysine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.4.3.2 AblA
-
 Methanosarcina mazei Go1
5.4.3.2 lysine-2,3-aminomutase
-
 Methanosarcina mazei Go1

Expression

EC Number Organism Comment Expression
5.4.3.2 Methanosarcina mazei Go1 a deletion mutant is constructed down

General Information

EC Number General Information Comment Organism
2.3.1.264 physiological function deletion of the genes encoding lysine-2,3-aminomutase (ablA) and beta-lysine acetyltransferase (ablB) abolishes the production of N6-acetyl-beta-lysine. The mutant grows well at low and intermediate salinities. Growth at 800 mM NaCl is only slowed down but not impaired. The mutant displays an increased glutamate pool and in addition, alanine is produced up to 0.36 micromol per mg protein. The cellular alanine concentration increases with the salinity of the medium and decreases in the presence of glycine betaine in the medium Methanosarcina mazei