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Literature summary extracted from

  • Rao, F.V.; Dorfmueller, H.C.; Villa, F.; Allwood, M.; Eggleston, I.M.; van Aalten, D.M.
    Structural insights into the mechanism and inhibition of eukaryotic O-GlcNAc hydrolysis (2006), EMBO J., 25, 1569-1578.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.169 cloned and overexpressed in Escherichia coli Clostridium perfringens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.169 significant sequence homology to the human O-GlcNAcase N-terminus Clostridium perfringens

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.169 D297N rather inactive mutant Clostridium perfringens
3.2.1.169 D298N rather inactive mutant Clostridium perfringens
3.2.1.169 D401A rather inactive mutant Clostridium perfringens
3.2.1.169 N390A as active as wild-type Clostridium perfringens
3.2.1.169 N396A rather inactive mutant Clostridium perfringens
3.2.1.169 W490A mutant with less activity Clostridium perfringens
3.2.1.169 Y335F rather inactive mutant Clostridium perfringens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.169 Ala-Cys(S-GlcNAc)-Ala pseudosubstrate Clostridium perfringens
3.2.1.169 O-(2-acetamido-2-deoxy-D-glucopyranosylidene)amino-N-phenylcarbamate PUGNAc, transition state mimic Clostridium perfringens
3.2.1.169 streptozotocin STZ, diabetogenic compound, acts as a suicide inhibitor Clostridium perfringens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.169 0.0029
-
 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide wild-type Clostridium perfringens
3.2.1.169 0.003
-
 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide D297N mutant Clostridium perfringens
3.2.1.169 0.0031
-
 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide N390A mutant Clostridium perfringens
3.2.1.169 0.004
-
 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide D298N mutant Clostridium perfringens
3.2.1.169 0.013
-
 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide N396A mutant Clostridium perfringens
3.2.1.169 0.015
-
 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide D401A mutant Clostridium perfringens
3.2.1.169 0.028
-
 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide W490A mutant Clostridium perfringens
3.2.1.169 0.041
-
 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide Y335F mutant Clostridium perfringens
3.2.1.169 0.121
-
 4-nitrophenyl N-acetyl-beta-D-glucosaminide
-
 Clostridium perfringens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.169 additional information Clostridium perfringens has O-GlcNAcase activity against O-GlcNAcylated human proteins, suggesting that the enzyme is a suitable model for further studies into the function of human O-GlcNAcase ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.169 Clostridium perfringens Q0TR53
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.169 glutathione affinity chromatography Clostridium perfringens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.169 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide + H2O
-
 Clostridium perfringens 4-methylumbelliferone + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.169 4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
-
 Clostridium perfringens 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.169 additional information has O-GlcNAcase activity against O-GlcNAcylated human proteins, suggesting that the enzyme is a suitable model for further studies into the function of human O-GlcNAcase Clostridium perfringens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.169 CpNagJ
-
 Clostridium perfringens
3.2.1.169 NagJ
-
 Clostridium perfringens
3.2.1.169 O-GlcNAcase
-
 Clostridium perfringens
3.2.1.169 OGA
-
 Clostridium perfringens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.169 0.0013
-
 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide D298N mutant Clostridium perfringens
3.2.1.169 0.0025
-
 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide N396A mutant Clostridium perfringens
3.2.1.169 0.0028
-
 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide Y335F mutant Clostridium perfringens
3.2.1.169 0.0044
-
 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide D401A mutant Clostridium perfringens
3.2.1.169 0.0198
-
 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide D297N mutant Clostridium perfringens
3.2.1.169 0.2
-
 4-nitrophenyl N-acetyl-beta-D-glucosaminide
-
 Clostridium perfringens
3.2.1.169 4
-
 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide W490A mutant Clostridium perfringens
3.2.1.169 10.5
-
 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide wild-type Clostridium perfringens
3.2.1.169 11
-
 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide N390A mutant Clostridium perfringens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.169 0.0000054
-
 O-(2-acetamido-2-deoxy-D-glucopyranosylidene)amino-N-phenylcarbamate
-
 Clostridium perfringens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.2.1.169 0.0000086
-
 wild-type Clostridium perfringens O-(2-acetamido-2-deoxy-D-glucopyranosylidene)amino-N-phenylcarbamate
3.2.1.169 0.00001
-
 N390A mutant Clostridium perfringens O-(2-acetamido-2-deoxy-D-glucopyranosylidene)amino-N-phenylcarbamate
3.2.1.169 0.000024
-
 W490A mutant Clostridium perfringens O-(2-acetamido-2-deoxy-D-glucopyranosylidene)amino-N-phenylcarbamate
3.2.1.169 0.0049
-
-
 Clostridium perfringens Ala-Cys(S-GlcNAc)-Ala
3.2.1.169 0.064
-
-
 Clostridium perfringens streptozotocin

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.169 0.0683
-
 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide Y335F mutant Clostridium perfringens
3.2.1.169 0.192
-
 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide N396A mutant Clostridium perfringens
3.2.1.169 0.293
-
 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide D401A mutant Clostridium perfringens
3.2.1.169 0.325
-
 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide D298N mutant Clostridium perfringens
3.2.1.169 6.6
-
 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide D297N mutant Clostridium perfringens
3.2.1.169 142.9
-
 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide W490A mutant Clostridium perfringens
3.2.1.169 3548
-
 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide N390A mutant Clostridium perfringens
3.2.1.169 3620
-
 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide wild-type Clostridium perfringens