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Literature summary extracted from

  • Krueger, S.K.; Henderson, M.C.; Siddens, L.K.; VanDyke, J.E.; Benninghoff, A.D.; Karplus, P.A.; Furnes, B.; Schlenk, D.; Williams, D.E.
    Characterization of sulfoxygenation and structural implications of human flavin-containing monooxygenase isoform 2 (FMO2.1) variants S195L and N413K (2009), Drug Metab. Dispos., 37, 1785-1791.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.13.8 cholate activates activity of mutant N413K by 23% Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.8 DNA sequence determination and analysis, and genotyping Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.8 N413K naturally occuring mutant of the FMO2*1 allele, the mutant shows higher kcat and Vmax, and increased thermosensitivity compared to the wild-type enzyme, activity is stabilized by NADPH Homo sapiens
1.14.13.8 Q472X naturally occuring mutant of the FMO2*1 allele, more frequent in the sub-Sahara African population Homo sapiens
1.14.13.8 S195L naturally occuring mutant of the FMO2*1 allele, the mutant shows reduced activity and increased pH sensitivity and thermosensitivity compared to the wild-type enzyme, activity is stabilized by NADPH Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.8 0.0048
-
 methyl p-tolyl sulfide pH 9.0, 37°C, mutant N413K Homo sapiens
1.14.13.8 0.005
-
 methyl p-tolyl sulfide pH 9.0, 37°C, mutant S195L Homo sapiens
1.14.13.8 0.0068
-
 NADPH pH 9.0, 37°C, mutant N413K Homo sapiens
1.14.13.8 0.0115
-
 NADPH pH 9.0, 37°C, wild-type FMO2.1 Homo sapiens
1.14.13.8 0.0135
-
 methyl p-tolyl sulfide pH 9.0, 37°C, wild-type FMO2.1 Homo sapiens
1.14.13.8 0.132
-
 NADPH pH 9.0, 37°C, mutant S195L Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.8 MgCl2 activates activity of mutant N413K by 18% Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.8 Homo sapiens Q99518
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.8 methyl p-tolyl sulfide + NADPH + H+ + O2
-
 Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.8 More structure comparison of wild-type and mutant enzymes, crystal structure analysis, overview Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.14.13.8 FMO2
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.8 37
-
 assay at Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.13.8 additional information
-
 loss of about 30% of activity after heat treatment of mutant enzyme N413K, activity is preservable by NADPH Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.13.8 10
-
 methyl p-tolyl sulfide pH 9.0, 37°C, mutant S195L Homo sapiens
1.14.13.8 15.2
-
 NADPH pH 9.0, 37°C, mutant S195L Homo sapiens
1.14.13.8 23.6
-
 NADPH pH 9.0, 37°C, wild-type FMO2.1 Homo sapiens
1.14.13.8 42.3
-
 methyl p-tolyl sulfide pH 9.0, 37°C, wild-type FMO2.1 Homo sapiens
1.14.13.8 71.1
-
 methyl p-tolyl sulfide pH 9.0, 37°C, mutant N413K Homo sapiens
1.14.13.8 94.4
-
 NADPH pH 9.0, 37°C, mutant N413K Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.8 9.5
-
 higher activity compared to pH 8.5 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.13.8 8.5 9.5
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.8 additional information FMO2 contains no bound FAD Homo sapiens
1.14.13.8 NADPH
-
 Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.14.13.8 0.00014
-
 NADPH pH 9.0, 37°C, mutant S195L Homo sapiens
1.14.13.8 0.0022
-
 methyl p-tolyl sulfide pH 9.0, 37°C, mutant S195L Homo sapiens
1.14.13.8 0.0033
-
 methyl p-tolyl sulfide pH 9.0, 37°C, wild-type FMO2.1 Homo sapiens
1.14.13.8 0.0068
-
 NADPH pH 9.0, 37°C, wild-type FMO2.1 Homo sapiens
1.14.13.8 0.0144
-
 NADPH pH 9.0, 37°C, mutant N413K Homo sapiens
1.14.13.8 0.0149
-
 methyl p-tolyl sulfide pH 9.0, 37°C, mutant N413K Homo sapiens