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Literature summary extracted from

  • Novick, R.M.; Elfarra, A.A.
    Purification and characterization of flavin-containing monooxygenase isoform 3 from rat kidney microsomes (2008), Drug Metab. Dispos., 36, 2468-2474.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.8 additional information
-
 additional information kinetics for kidney and liver enzymes, overview Rattus norvegicus
1.14.13.8 0.01
-
 Methimazole pH 7.6, 37°C, purified FMO3 from liver Rattus norvegicus
1.14.13.8 0.04
-
 Methimazole pH 7.6, 37°C, purified FMO3 from kidney Rattus norvegicus
1.14.13.8 0.31
-
 seleno-L-methionine pH 7.6, 37°C, purified FMO3 from liver Rattus norvegicus
1.14.13.8 0.35
-
 seleno-L-methionine pH 7.6, 37°C, purified FMO3 from kidney Rattus norvegicus
1.14.13.8 2.8
-
 L-methionine pH 7.6, 37°C, purified FMO3 from kidney Rattus norvegicus
1.14.13.8 3.4
-
 L-methionine pH 7.6, 37°C, purified FMO3 from liver Rattus norvegicus
1.14.13.8 4.3
-
 S-allyl-L-cysteine pH 7.6, 37°C, purified FMO3 from kidney Rattus norvegicus
1.14.13.8 5.9
-
 S-allyl-L-cysteine pH 7.6, 37°C, purified FMO3 from liver Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.13.8 microsome
-
 Rattus norvegicus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.8 56000
-
 x * 56000, kidney enzyme, SDS-PAGE Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.8 Rattus norvegicus Q9EQ76 male sprague-dawley rats
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.8 native FMO3 130fold from kidney microsomes Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.8 kidney
-
 Rattus norvegicus
-
1.14.13.8 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.8 0.15
-
 purified enzyme, substrate L-methionine Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.8 L-methionine + NADPH + H+ + O2 stereochemistry, overview Rattus norvegicus methionine S-oxide + NADP+ + H2O formation of 80% D-isomer ?
1.14.13.8 methimazole + NADPH + H+ + O2 i.e. N-methyl-2-mercaptoimidazole Rattus norvegicus methimazole S-oxide + NADP+ + H2O
-
 ?
1.14.13.8 S-allyl-L-cysteine + NADPH + H+ + O2 stereochemmistry, overview Rattus norvegicus ? + NADP+ + H2O
-
 ?
1.14.13.8 seleno-L-methionine + NADPH + H+ + O2
-
 Rattus norvegicus seleno-L-methionine Se-oxide + NADP+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.8 ? x * 56000, kidney enzyme, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
1.14.13.8 FMO3
-
 Rattus norvegicus
1.14.13.8 Met S-oxidase
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.8 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.8 7.6
-
 assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.8 FAD consensus binding sequence is Gly-Xaa-Gly-Xaa-Xaa-Gly Rattus norvegicus
1.14.13.8 NADPH consensus binding sequence is Gly-Leu-Gly-Asn-Ser-Gly Rattus norvegicus

General Information

EC Number General Information Comment Organism
1.14.13.8 physiological function FMO3 plays an important role in kidney metabolism of xenobiotics containing sulfur and selenium atoms Rattus norvegicus