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Literature summary extracted from

  • Okafuji, A.; Biskup, T.; Hitomi, K.; Getzoff, E.D.; Kaiser, G.; Batschauer, A.; Bacher, A.; Hidema, J.; Teranishi, M.; Yamamoto, K.; Schleicher, E.; Weber, S.
    Light-induced activation of class II cyclobutane pyrimidine dimer photolyases (2010), DNA Repair, 9, 495-505.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.99.3 expressed in Escherichia coli Codon Plus BL21[pREP4] cells Arabidopsis thaliana
4.1.99.3 GST-tagged class II CPD photolyase overexpressed in Escherichia coli Oryza sativa

Protein Variants

EC Number Protein Variants Comment Organism
4.1.99.3 E109A is catalytically active but unable to bind the second cofactor methenyltetrahydrofolate Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
4.1.99.3 Arabidopsis thaliana
-
-
-
4.1.99.3 Escherichia coli
-
-
-
4.1.99.3 Oryza sativa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.99.3
-
 Arabidopsis thaliana
4.1.99.3
-
 Oryza sativa

Storage Stability

EC Number Storage Stability Organism
4.1.99.3 -70°C, 0.05 M sodium phosphate, pH 7.0, 0.1 M NaCl, 20% (v/v) glycerol Escherichia coli
4.1.99.3 -70°C, 0.05 M sodium phosphate, pH 7.0, 0.1 M NaCl, 20% (v/v) glycerol Arabidopsis thaliana
4.1.99.3 -70°C, 0.05 M sodium phosphate, pH 7.0, 0.1 M NaCl, 20% (v/v) glycerol Oryza sativa

Synonyms

EC Number Synonyms Comment Organism
4.1.99.3 class I CPD photolyase
-
 Escherichia coli
4.1.99.3 class I cyclobutane pyrimidine dimer photolyase
-
 Escherichia coli
4.1.99.3 class I photolyase
-
 Escherichia coli
4.1.99.3 class II CPD photolyase
-
 Arabidopsis thaliana
4.1.99.3 class II CPD photolyase
-
 Oryza sativa
4.1.99.3 class II cyclobutane pyrimidine dimer photolyase
-
 Arabidopsis thaliana
4.1.99.3 class II cyclobutane pyrimidine dimer photolyase
-
 Oryza sativa
4.1.99.3 class II photolyase
-
 Arabidopsis thaliana
4.1.99.3 class II photolyase
-
 Oryza sativa
4.1.99.3 CPD photolyase
-
 Escherichia coli
4.1.99.3 CPD photolyase
-
 Arabidopsis thaliana
4.1.99.3 CPD photolyase
-
 Oryza sativa
4.1.99.3 CPDI
-
 Escherichia coli
4.1.99.3 CPDII
-
 Arabidopsis thaliana
4.1.99.3 CPDII
-
 Oryza sativa

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.99.3 FAD
-
 Escherichia coli
4.1.99.3 FAD binds the flavin cofactor in a pocket that is conserved in terms of its electronic properties Arabidopsis thaliana
4.1.99.3 FAD binds the flavin cofactor in a pocket that is conserved in terms of its electronic properties. W399-W378-W406 may function as potential electron donors to the flavin and are possible candidate tryptophans for light-induced electron transfer Oryza sativa
4.1.99.3 FADH2 photoreduction of FADH proceeds along the conserved tryptophan triad W306-W359-W382 Escherichia coli
4.1.99.3 methenyltetrahydrofolate
-
 Escherichia coli

General Information

EC Number General Information Comment Organism
4.1.99.3 metabolism photoreduction kinetics of class II photolyases are very similar to those of the other classes with even higher photoreduction rates in class II as compared to class I. W399-W378-W406 electron-transfer pathway is conserved among class II CPD photolyase enzymes Arabidopsis thaliana
4.1.99.3 metabolism photoreduction kinetics of class II photolyases are very similar to those of the other classes with even higher photoreduction rates in class II as compared to class I. W399-W378-W406 electron-transfer pathway is conserved among class II CPD photolyase enzymes Oryza sativa