EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.45 | 5-fluorodeoxyuridine | - |
Homo sapiens | |
2.1.1.45 | raltitrexed | - |
Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.45 | Homo sapiens | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.1.1.45 | HeLa cell | - |
Homo sapiens | - |
2.1.1.45 | HT-29 cell | - |
Homo sapiens | - |
2.1.1.45 | MCF-7 cell | - |
Homo sapiens | - |
2.1.1.45 | MDA-MB-231 cell | - |
Homo sapiens | - |
2.1.1.45 | MSTO-211H cell | - |
Homo sapiens | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.45 | 5,10-methylenetetrahydrofolate + 6-fluoro-dUMP | - |
Homo sapiens | ? | - |
? | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | - |
Homo sapiens | dihydrofolate + dTMP | - |
? |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.45 | N5,N10-methylenetetrahydrofolate | - |
Homo sapiens |
EC Number | Organism | Comment | Expression |
---|---|---|---|
2.1.1.45 | Homo sapiens | thymidylate synthase antisense oligodeoxynucleotide (ODN) 491 decreases thymidylate protein levels in HeLa cells with a concomitant increase in sensitivity to thymidylate synthase-targeting chemotherapeutics. In MCF-7 cells, ODN491 treatment is less effective at reducing thymidylate synthase mRNA and does not reduce thymidylate synthase protein, nor does it enhance sensitivity to thymidylate synthase-targeting or other chemotherapeutics. Treatment with either ODN 83 or ODN 491 reduces 6-fluoro-dUMP binding in HeLa cells by 59% and 62%, respectively, compared with control | down |