Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Paschal, B.M.; McReynolds, L.A.; Noren, C.J.; Nichols, N.M.
    RNA polymerases (2008), Curr. Protoc. Mol. Biol., Chapter 3, Unit3.8.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.7.6 synthesis the enzyme is useful for in vitro transcription reactions to produce preparative quantities of transcribed RNA and labeled RNA probes, method evaluation, overview Escherichia coli
2.7.7.6 synthesis the enzyme is useful for in vitro transcription reactions to produce preparative quantities of transcribed RNA and labeled RNA probes, transcripts thousands of nucleotides in length, as are the major applications of these reactions, method evaluation, overview. Phage T7 RNA polymerase is an extremely processive enzyme in high-yield transcription of DNA sequences inserted downstream from the corresponding T7 promoter, applications overview Escherichia phage T7
2.7.7.6 synthesis the enzyme is useful for in vitro transcription reactions to produce preparative quantities of transcribed RNAand labeled RNA probes, transcripts thousands of nucleotides in length, as are the major applications of these reactions, method evaluation, overview. Phage SP6 RNA polymerase is an extremely processive enzyme in high-yield transcription of DNA sequences inserted downstream from the corresponding SP6 promoter, applications overview Zindervirus SP6
2.7.7.6 synthesis the enzyme is useful for in vitro transcription reactions to produce preparative quantities of transcribed RNAand labeled RNA probes, transcripts thousands of nucleotides in length, as are the major applications of these reactions, method evaluation, overview. Phage T3 RNA polymerase is an extremely processive enzyme in high-yield transcription of DNA sequences inserted downstream from the corresponding T3 promoter, applications overview Enterobacteria phage T3
2.7.7.48 synthesis RdRp from the bacteriophage phi6 can be used to generate dsRNA for RNA interference, RNAi, applications, resulting in in vivo gene silencing. RNA molecules that are radioactively or fluorescently labeled using poly(A) polymerase can be used as probes in a wide variety of applications Cystovirus phi6

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.6
-
 Escherichia coli
2.7.7.6 construction of plasmids with polylinker cloning sites adjacent to the RNA polymerase promoter Escherichia phage T7
2.7.7.6 construction of plasmids with polylinker cloning sites adjacent to the RNA polymerase promoter Enterobacteria phage T3
2.7.7.6 construction of plasmids with polylinker cloning sites adjacent to the RNA polymerase promoter Zindervirus SP6

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.6 K+ activates Escherichia coli
2.7.7.6 Mg2+ required Escherichia coli
2.7.7.6 Mg2+ required Escherichia phage T7
2.7.7.6 Mg2+ required Enterobacteria phage T3
2.7.7.6 Mg2+ required Zindervirus SP6
2.7.7.48 Mn2+
-
 Cystovirus phi6

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.6 480000
-
 about Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.6 nucleoside triphosphate + RNAn Escherichia coli template is DNA diphosphate + RNAn+1
-
 ?
2.7.7.6 nucleoside triphosphate + RNAn Escherichia phage T7 template is DNA diphosphate + RNAn+1
-
 ?
2.7.7.6 nucleoside triphosphate + RNAn Enterobacteria phage T3 template is DNA diphosphate + RNAn+1
-
 ?
2.7.7.6 nucleoside triphosphate + RNAn Zindervirus SP6 template is DNA diphosphate + RNAn+1
-
 ?
2.7.7.48 nucleoside triphosphate + RNAn Cystovirus phi6 template is RNA, the phi6 polymerase is highly processive and can use either single- or double-stranded RNA as a template and synthesizes a full-length complementary strand of an RNA diphosphate + RNAn+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.6 Enterobacteria phage T3
-
-
-
2.7.7.6 Escherichia coli
-
-
-
2.7.7.6 Escherichia phage T7
-
-
-
2.7.7.6 Zindervirus SP6
-
-
-
2.7.7.48 Cystovirus phi6
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.6 additional information the core enzyme, which lacks the sigma subunit, synthesizes short transcripts relatively uniformly on the DNA template in the presence of high concentrations of random primers and low NTP concentrations Escherichia coli ?
-
 ?
2.7.7.6 nucleoside triphosphate + RNAn template is DNA Escherichia coli diphosphate + RNAn+1
-
 ?
2.7.7.6 nucleoside triphosphate + RNAn template is DNA Escherichia phage T7 diphosphate + RNAn+1
-
 ?
2.7.7.6 nucleoside triphosphate + RNAn template is DNA Enterobacteria phage T3 diphosphate + RNAn+1
-
 ?
2.7.7.6 nucleoside triphosphate + RNAn template is DNA Zindervirus SP6 diphosphate + RNAn+1
-
 ?
2.7.7.6 nucleoside triphosphate + RNAn template is DNA, native or denatured, method evaluation: specificity and extent of transcription depends strongly on the quality of the DNA preparation, the strength of the promoter and terminator sequences, and the kind and concentration of mono- and divalent cations in the reaction mixture Escherichia coli diphosphate + RNAn+1
-
 ?
2.7.7.48 nucleoside triphosphate + RNAn template is RNA, the phi6 polymerase is highly processive and can use either single- or double-stranded RNA as a template and synthesizes a full-length complementary strand of an RNA Cystovirus phi6 diphosphate + RNAn+1
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.6 pentamer subunits structure alpha2betabeta'sigmaomega Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.7.7.6 DNA-dependent RNA polymerase
-
 Escherichia coli
2.7.7.6 DNA-dependent RNA polymerase
-
 Escherichia phage T7
2.7.7.6 DNA-dependent RNA polymerase
-
 Enterobacteria phage T3
2.7.7.6 DNA-dependent RNA polymerase
-
 Zindervirus SP6
2.7.7.48 phi6 polymerase
-
 Cystovirus phi6
2.7.7.48 RDRP
-
 Cystovirus phi6
2.7.7.48 RNA-dependent RNA polymerase
-
 Cystovirus phi6

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.6 37
-
 assay at Escherichia coli
2.7.7.6 37
-
 assay at Escherichia phage T7
2.7.7.6 37
-
 assay at Enterobacteria phage T3
2.7.7.6 37
-
 assay at Zindervirus SP6
2.7.7.48 32
-
 assay at Cystovirus phi6

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.6 7.5
-
 assay at Escherichia phage T7
2.7.7.6 7.5
-
 assay at Enterobacteria phage T3
2.7.7.6 7.5
-
 assay at Zindervirus SP6
2.7.7.6 8
-
 assay at Escherichia coli
2.7.7.48 8.9
-
 assay at Cystovirus phi6

General Information

EC Number General Information Comment Organism
2.7.7.48 physiological function RdRps are unique in that they create dsRNA by initiating polymerization at the 3' end of the substrate Cystovirus phi6