Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Himes, R.A.; Karlin, K.D.
    Copper-dioxygen complex mediated C-H bond oxygenation: relevance for particulate methane monooxygenase (pMMO) (2009), Curr. Opin. Chem. Biol., 13, 119-131.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.18.3
-
 Methylosinus trichosporium OB3b
1.14.18.3 heterotrimer of three subunits, pmoA, pmoB, and pmoC, with a solvent-exposed domain above the trans-membrane domain, which consists of alpha-helices Methylococcus capsulatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.18.3 copper two-coordinate (His2) mononuclear copper site and an anomalous site modeled as a dinuclear copper cluster. The cluster has one Cu ion bound by two His imidazoles and another by an imidazole and amino group of the pmoB N-terminal His Methylococcus capsulatus
1.14.18.3 Zinc a zinc site, probably adventitious, as the crystallization medium requires the metal. Purified pMMO does not contain zinc in the trans-membrane domain Methylococcus capsulatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.18.3 membrane
-
 Methylococcus capsulatus 16020
-
1.14.18.3 membrane
-
 Methylosinus trichosporium OB3b 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.18.3 copper an anomalous site modeled as a dinuclear copper cluster. The mononuclear copper site is absent (one His is not conserved), and the zinc replaced by a copper ion Methylosinus trichosporium OB3b
1.14.18.3 additional information purified pMMO does not contain zinc in the trans-membrane domain Methylosinus trichosporium OB3b

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.3 Methylococcus capsulatus
-
-
-
1.14.18.3 Methylococcus capsulatus Bath
-
-
-
1.14.18.3 Methylosinus trichosporium OB3b
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.3 methane + reduced acceptor + H* + O2
-
 Methylococcus capsulatus methanol + acceptor + H2O
-
 ?
1.14.18.3 methane + reduced acceptor + H* + O2
-
 Methylosinus trichosporium OB3b methanol + acceptor + H2O
-
 ?
1.14.18.3 methane + reduced acceptor + H* + O2
-
 Methylococcus capsulatus Bath methanol + acceptor + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.18.3 heterotrimer crystallography Methylococcus capsulatus

Synonyms

EC Number Synonyms Comment Organism
1.14.18.3 particulate methane monooxygenase
-
 Methylococcus capsulatus
1.14.18.3 particulate methane monooxygenase
-
 Methylosinus trichosporium OB3b
1.14.18.3 pMMO
-
 Methylococcus capsulatus
1.14.18.3 pMMO
-
 Methylosinus trichosporium OB3b

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.18.3 NADH
-
 Methylococcus capsulatus
1.14.18.3 NADH
-
 Methylosinus trichosporium OB3b
1.14.18.3 NADPH
-
 Methylococcus capsulatus
1.14.18.3 NADPH
-
 Methylosinus trichosporium OB3b