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Literature summary extracted from

  • Lyu, R.C.; Hu, H.Y.; Kuo, L.Y.; Lo, H.F.; Ong, P.L.; Chang, H.P.; Lin, L.L.
    Role of the conserved Thr399 and Thr417 residues of Bacillus licheniformis gamma-Glutamyltranspeptidase as evaluated by mutational analysis (2009), Curr. Microbiol., 59, 101-106.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.3.2.2 T399A complete loss of enzymatic activity Bacillus licheniformis
2.3.2.2 T399C complete loss of enzymatic activity Bacillus licheniformis
2.3.2.2 T399S dramatic reduction in activity Bacillus licheniformis
2.3.2.2 T399S precursor is able to precede a time-dependent autocatalytic process to generate the 44.9 and 21.7 kDa subunits of mature enzyme Bacillus licheniformis
2.3.2.2 T417A complete loss of enzymatic activity Bacillus licheniformis
2.3.2.2 T417A precursor is able to precede a time-dependent autocatalytic process to generate the 44.9 and 21.7 kDa subunits of mature enzyme, processed enzyme lacks activity Bacillus licheniformis
2.3.2.2 T417K complete loss of enzymatic activity Bacillus licheniformis
2.3.2.2 T417S dramatic reduction in activity Bacillus licheniformis
2.3.2.2 T417S precursor is able to precede a time-dependent autocatalytic process to generate the 44.9 and 21.7 kDa subunits of mature enzyme Bacillus licheniformis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.2.2 0.07
-
 5-L-glutamyl-4-nitroanilide wild-type, pH 8.0, 40°C Bacillus licheniformis
2.3.2.2 0.09
-
 5-L-glutamyl-4-nitroanilide mutant T417S, pH 8.0, 40°C Bacillus licheniformis
2.3.2.2 0.11
-
 5-L-glutamyl-4-nitroanilide mutant T399S, pH 8.0, 40°C Bacillus licheniformis

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.2 Bacillus licheniformis
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.2.2 1.25
-
 mutant T399S, pH 8.0, 40°C Bacillus licheniformis
2.3.2.2 12.7
-
 mutant T417S, pH 8.0, 40°C Bacillus licheniformis
2.3.2.2 83.7
-
 wild-type, pH 8.0, 40°C Bacillus licheniformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.2 5-L-glutamyl-4-nitroanilide + Gly-Gly
-
 Bacillus licheniformis 4-nitroaniline + 5-L-glutamyl-Gly-Gly
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.2.2 1.42
-
 5-L-glutamyl-4-nitroanilide mutant T399S, pH 8.0, 40°C Bacillus licheniformis
2.3.2.2 24.24
-
 5-L-glutamyl-4-nitroanilide mutant T417S, pH 8.0, 40°C Bacillus licheniformis
2.3.2.2 109.5
-
 5-L-glutamyl-4-nitroanilide wild-type, pH 8.0, 40°C Bacillus licheniformis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.2.2 13
-
 5-L-glutamyl-4-nitroanilide mutant T399S, pH 8.0, 40°C Bacillus licheniformis
2.3.2.2 269.5
-
 5-L-glutamyl-4-nitroanilide mutant T417S, pH 8.0, 40°C Bacillus licheniformis
2.3.2.2 1504
-
 5-L-glutamyl-4-nitroanilide wild-type, pH 8.0, 40°C Bacillus licheniformis