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Literature summary extracted from

  • Fukui, H.; Hanaoka, R.; Kawahara, A.
    Noncanonical activity of seryl-tRNA synthetase is involved in vascular development (2009), Circ. Res., 104, 1253-1259.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.11 into the vector pGEX6p3 for expression in Escherichia coli cells Danio rerio

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.11 additional information ko095, an embryonic lethal mutant with abnormal branching in the head and trunk blood vessels, the gene responsible for ko095 encodes seryl-tRNA synthetase with a nonsense mutation Danio rerio
6.1.1.11 T429A the abnormal branching of intersegmental vessels in ko095 mutant is suppressed by the introduction of either wild-type or mutant T429A seryl-tRNA synthetase, T429A lacks the enzymatic activity that catalyzes aminoacylation of transfer RNA for serine Danio rerio

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.11 ATP + L-serine + tRNASer Danio rerio
-
 AMP + diphosphate + L-seryl-tRNASer
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.11 Danio rerio Q6DRC0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.11 by glutathione Sepharose beads Danio rerio

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.11 ATP + L-serine + tRNASer
-
 Danio rerio AMP + diphosphate + L-seryl-tRNASer
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.11 Seryl-tRNA synthetase
-
 Danio rerio

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.11 22
-
 aminoacylation assay at room temperature Danio rerio

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.11 7.5
-
 aminoacylation assay Danio rerio

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.11 ATP
-
 Danio rerio

General Information

EC Number General Information Comment Organism
6.1.1.11 physiological function seryl-tRNA synthetase is involved in vascular development Danio rerio