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Literature summary extracted from

  • Deng, H.; Cross, S.M.; McGlinchey, R.P.; Hamilton, J.T.; OHagan, D.
    In vitro reconstituted biotransformation of 4-fluorothreonine from fluoride ion: application of the fluorinase (2008), Chem. Biol., 15, 1268-1276.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.5.1.63 biotechnology fluorochemical production (18F-labelled organic compounds) for medicinal chemistry research (positron emission tomography), when enzyme acts together with other enzymes in the fluorometabolite production (purine nucleoside phosphorylase, isomerase, aldolase, enzyme generating fluoroacetaldehyde in a retro-aldol reaction, fluoroacetaldehyde dehydrogenase or pyridoxal phosphate-dependent enzyme) to generate fluoroacetaldehyde and 4-fluorothreonine Streptomyces cattleya

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.2.1.8 expression in Escherichia coli BL21, transfection to Streptococcus lividans TK24 Streptomyces cattleya
2.4.2.1 expression in Escherichia coli BL21 Streptomyces cattleya
2.5.1.63 expression in Escherichia coli BL21 Streptomyces cattleya
2.5.1.63 expression in Escherichia coli BL21(DE3) Streptomyces cattleya
4.1.2.13 expression in Escherichia coli Streptomyces coelicolor

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.2.13 Zn2+ dependent Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.2.1.8 L-threonine + fluoroacetaldehyde Streptomyces cattleya
-
4-fluoro-L-threonine + acetaldehyde
-
?
2.5.1.63 S-adenosyl-L-methionine + fluoride Streptomyces cattleya
-
5'-deoxy-5'-fluoroadenosine + L-methionine
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.8 Streptomyces cattleya B6VP39
-
-
2.4.2.1 Streptomyces cattleya
-
-
-
2.5.1.63 Streptomyces cattleya
-
-
-
4.1.2.13 Streptomyces coelicolor
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.2.1.8 partially by Ni-affinity column, size exclusion chromatography Streptomyces cattleya
2.4.2.1
-
Streptomyces cattleya
2.5.1.63
-
Streptomyces cattleya
4.1.2.13
-
Streptomyces coelicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.8 L-threonine + fluoroacetaldehyde
-
Streptomyces cattleya 4-fluoro-L-threonine + acetaldehyde
-
?
2.5.1.63 S-adenosyl-L-methionine + fluoride
-
Streptomyces cattleya 5'-deoxy-5'-fluoroadenosine + L-methionine
-
?
4.1.2.13 5-fluoro-5-deoxy-D-ribulose 1-phosphate
-
Streptomyces coelicolor fluoroacetaldehyde + dihydroxyacetone phosphate
-
r

Synonyms

EC Number Synonyms Comment Organism
2.2.1.8 4-fluorothreoinine transaldolase
-
Streptomyces cattleya
2.2.1.8 4-FTase
-
Streptomyces cattleya
2.4.2.1 flB
-
Streptomyces cattleya
2.4.2.1 PNP
-
Streptomyces cattleya
2.4.2.1 purine nucleoside phosphorylase
-
Streptomyces cattleya
2.5.1.63 5'-fluoro-5'-deoxyadenosine synthase
-
Streptomyces cattleya
2.5.1.63 flA
-
Streptomyces cattleya
2.5.1.63 flA protein
-
Streptomyces cattleya
2.5.1.63 fluorinase
-
Streptomyces cattleya
4.1.2.13 fuculose aldolase
-
Streptomyces coelicolor

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.2.1.8 37
-
assay at Streptomyces cattleya

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.8 pyridoxal 5'-phosphate
-
Streptomyces cattleya

General Information

EC Number General Information Comment Organism
2.2.1.8 physiological function final step of 4-fluorothreonine biosynthesis Streptomyces cattleya
2.4.2.1 physiological function part of 4-fluorothreonine biosynthesis Streptomyces cattleya
2.5.1.63 metabolism first enzyme in the fluorometabolite pathway to produce the mammalian toxin fluoroacetate and the antibiotic 4-fluorothreonine Streptomyces cattleya
2.5.1.63 physiological function part of 4-fluorothreonine biosynthesis Streptomyces cattleya
4.1.2.13 physiological function part of 4-fluorothreonine biosynthesis Streptomyces coelicolor