EC Number | Application | Comment | Organism |
---|---|---|---|
2.5.1.63 | biotechnology | fluorochemical production (18F-labelled organic compounds) for medicinal chemistry research (positron emission tomography), when enzyme acts together with other enzymes in the fluorometabolite production (purine nucleoside phosphorylase, isomerase, aldolase, enzyme generating fluoroacetaldehyde in a retro-aldol reaction, fluoroacetaldehyde dehydrogenase or pyridoxal phosphate-dependent enzyme) to generate fluoroacetaldehyde and 4-fluorothreonine | Streptomyces cattleya |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.2.1.8 | expression in Escherichia coli BL21, transfection to Streptococcus lividans TK24 | Streptomyces cattleya |
2.4.2.1 | expression in Escherichia coli BL21 | Streptomyces cattleya |
2.5.1.63 | expression in Escherichia coli BL21 | Streptomyces cattleya |
2.5.1.63 | expression in Escherichia coli BL21(DE3) | Streptomyces cattleya |
4.1.2.13 | expression in Escherichia coli | Streptomyces coelicolor |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.1.2.13 | Zn2+ | dependent | Streptomyces coelicolor |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.2.1.8 | L-threonine + fluoroacetaldehyde | Streptomyces cattleya | - |
4-fluoro-L-threonine + acetaldehyde | - |
? | |
2.5.1.63 | S-adenosyl-L-methionine + fluoride | Streptomyces cattleya | - |
5'-deoxy-5'-fluoroadenosine + L-methionine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.2.1.8 | Streptomyces cattleya | B6VP39 | - |
- |
2.4.2.1 | Streptomyces cattleya | - |
- |
- |
2.5.1.63 | Streptomyces cattleya | - |
- |
- |
4.1.2.13 | Streptomyces coelicolor | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.2.1.8 | partially by Ni-affinity column, size exclusion chromatography | Streptomyces cattleya |
2.4.2.1 | - |
Streptomyces cattleya |
2.5.1.63 | - |
Streptomyces cattleya |
4.1.2.13 | - |
Streptomyces coelicolor |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.2.1.8 | L-threonine + fluoroacetaldehyde | - |
Streptomyces cattleya | 4-fluoro-L-threonine + acetaldehyde | - |
? | |
2.5.1.63 | S-adenosyl-L-methionine + fluoride | - |
Streptomyces cattleya | 5'-deoxy-5'-fluoroadenosine + L-methionine | - |
? | |
4.1.2.13 | 5-fluoro-5-deoxy-D-ribulose 1-phosphate | - |
Streptomyces coelicolor | fluoroacetaldehyde + dihydroxyacetone phosphate | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.2.1.8 | 4-fluorothreoinine transaldolase | - |
Streptomyces cattleya |
2.2.1.8 | 4-FTase | - |
Streptomyces cattleya |
2.4.2.1 | flB | - |
Streptomyces cattleya |
2.4.2.1 | PNP | - |
Streptomyces cattleya |
2.4.2.1 | purine nucleoside phosphorylase | - |
Streptomyces cattleya |
2.5.1.63 | 5'-fluoro-5'-deoxyadenosine synthase | - |
Streptomyces cattleya |
2.5.1.63 | flA | - |
Streptomyces cattleya |
2.5.1.63 | flA protein | - |
Streptomyces cattleya |
2.5.1.63 | fluorinase | - |
Streptomyces cattleya |
4.1.2.13 | fuculose aldolase | - |
Streptomyces coelicolor |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.2.1.8 | 37 | - |
assay at | Streptomyces cattleya |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.2.1.8 | pyridoxal 5'-phosphate | - |
Streptomyces cattleya |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.2.1.8 | physiological function | final step of 4-fluorothreonine biosynthesis | Streptomyces cattleya |
2.4.2.1 | physiological function | part of 4-fluorothreonine biosynthesis | Streptomyces cattleya |
2.5.1.63 | metabolism | first enzyme in the fluorometabolite pathway to produce the mammalian toxin fluoroacetate and the antibiotic 4-fluorothreonine | Streptomyces cattleya |
2.5.1.63 | physiological function | part of 4-fluorothreonine biosynthesis | Streptomyces cattleya |
4.1.2.13 | physiological function | part of 4-fluorothreonine biosynthesis | Streptomyces coelicolor |