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Literature summary extracted from
- Structural characterization of the catalytic calcium-binding site in diisopropyl fluorophosphatase (DFPase)-Comparison with related beta-propeller enzymes (2010), Chem. Biol. Interact., 187, 373-379.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.8.2 | expressed in Escherichia coli BL21(DE3) cells | Loligo vulgaris |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.1.8.2 | mutant enzymes N120D/N175D/D229N, E21QN120D/N175D/D229N, and D121E are crystallized by hanging drop vapor diffusion method, using 0.1 M MES buffer pH 6.5, 14-20% (w/v) PEG 3350 | Loligo vulgaris |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.1.8.2 | D121E | the mutant displays 87% activity compared to the wild type enzyme | Loligo vulgaris |
| 3.1.8.2 | D229N | enzymatically inactive | Loligo vulgaris |
| 3.1.8.2 | E21Q/N120D/N175D/D229N | the mutations lead to a loss of calcium binding and enzymatic activity | Loligo vulgaris |
| 3.1.8.2 | N120D/N175D/D229N | the mutations lead to a loss of calcium binding and enzymatic activity | Loligo vulgaris |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.8.2 | Ca2+ | required for activity, the wild type enzyme contains two Ca2+ ions | Loligo vulgaris |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.8.2 | Loligo vulgaris | Q7SIG4 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.8.2 | Ni-NTA column chromatography and Q-Sepharose column chromatography | Loligo vulgaris |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.8.2 | diisopropyl fluorophosphate + H2O | - |
Loligo vulgaris | diisopropyl phosphate + fluoride | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.8.2 | DFPase | - |
Loligo vulgaris |
| 3.1.8.2 | diisopropyl fluorophosphatase | - |
Loligo vulgaris |
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Release 2023.1 (January 2023)
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