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Literature summary extracted from

  • Warby, C.A.; Phillips, J.D.; Bergonia, H.A.; Whitby, F.G.; Hill, C.P.; Kushner, J.P.
    Structural and kinetic characterization of mutant human uroporphyrinogen decarboxylases (2009), Cell. Mol. Biol. (Noisy-le-grand), 55, 40-45.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.37 uroporphyrinogen decarboxylase mutations G318R, D306Y and K297N Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.1.37 recombinant mutant proteins K297N and G318R Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.37 D306Y mutation results in an insoluble recombinant protein Homo sapiens
4.1.1.37 G318R little effect on the structure or activity of recombinant uroporphyrinogen decarboxylase, but the protein displays reduced stability in vitro Homo sapiens
4.1.1.37 K297N little effect on the structure or activity of recombinant uroporphyrinogen decarboxylase, but the protein displays reduced stability in vitro Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.37 0.0108
-
 uroporphyrinogen-III recombinant wild-type Homo sapiens
4.1.1.37 0.0144
-
 uroporphyrinogen-III recombinant mutant K297N Homo sapiens
4.1.1.37 0.0148
-
 uroporphyrinogen-III recombinant mutant G318R Homo sapiens
4.1.1.37 0.0277
-
 uroporphyrinogen-I recombinant mutant G318R Homo sapiens
4.1.1.37 0.033
-
 uroporphyrinogen-I recombinant mutant K297N Homo sapiens
4.1.1.37 0.0381
-
 uroporphyrinogen-I recombinant wild-type Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.37 uroporphyrinogen-III Homo sapiens
-
 coproporphyrinogen-III + 4 CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.37 Homo sapiens P06132
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.37
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.1.37 hepatocyte
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.37 additional information
-
 recombinat mutant G318R substrate uroporphyrinogen-I 60.9 percent of recombinant wild-type Homo sapiens
4.1.1.37 additional information
-
 recombinat mutant G318R substrate uroporphyrinogen-III 76.2 percent of recombinant wild-type Homo sapiens
4.1.1.37 additional information
-
 recombinat mutant K297N substrate uroporphyrinogen-I 63.1 percent of recombinant wild-type Homo sapiens
4.1.1.37 additional information
-
 recombinat mutant K297N substrate uroporphyrinogen-III 73.7 percent of recombinant wild-type Homo sapiens
4.1.1.37 additional information
-
 subnormal uroporphyrinogen decarboxylase activity results in accumulation and urinary excretion of uroporphyrin and heptacarboxyl porphyrin Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.37 uroporphyrinogen-I
-
 Homo sapiens coproporphyrinogen-I + 4 CO2
-
 ?
4.1.1.37 uroporphyrinogen-III
-
 Homo sapiens coproporphyrinogen-III + 4 CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.37 UroD
-
 Homo sapiens
4.1.1.37 uroporphyrinogen decarboxylase
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.37 0.102
-
 uroporphyrinogen-I recombinant mutant G318R Homo sapiens
4.1.1.37 0.104
-
 uroporphyrinogen-I recombinant mutant K297N Homo sapiens
4.1.1.37 0.124
-
 uroporphyrinogen-I recombinant wild-type Homo sapiens
4.1.1.37 0.317
-
 uroporphyrinogen-III recombinant wild-type Homo sapiens
4.1.1.37 0.361
-
 uroporphyrinogen-III recombinant mutant K297N Homo sapiens
4.1.1.37 0.425
-
 uroporphyrinogen-III recombinant mutant G318R Homo sapiens