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Literature summary extracted from

  • Ibrahim-Granet, O.; Dubourdeau, M.; Latgé, J.; Ave, P.; Huerre, M.; Brakhage, A.; Brock, M.
    Methylcitrate synthase from Aspergillus fumigatus is essential for manifestation of invasive aspergillosis (2008), Cell. Microbiol., 10, 134-148.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.3.5 medicine invasive aspergillosis is a life-threatening disease mainly caused by the fungus Aspergillus fumigatus, blocking of methylcitrate synthase abrogates fungal growth and provides a suitable target for new antifungals Aspergillus fumigatus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.3.5 into the pCR2.1 and pUChph1 vectors Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.5 propionyl-CoA + H2O + oxaloacetate Aspergillus fumigatus
-
 (2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.5 Aspergillus fumigatus
-
 wild-type strain CBS 144.89, methylcitrate synthase mutant DELTAmcsA, complemented methylcitrate synthase mutant 11/12 B1
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.3.5 0.003
-
 wild-type strain, carbon source glucose Aspergillus fumigatus
2.3.3.5 0.004
-
 complemented methylcitrate synthase mutant 11/12 B1, carbon source glucose Aspergillus fumigatus
2.3.3.5 0.011
-
 wild-type strain, carbon source olive oil Aspergillus fumigatus
2.3.3.5 0.029
-
 complemented methylcitrate synthase mutant 11/12 B1, carbon source olive oil Aspergillus fumigatus
2.3.3.5 0.085
-
 wild-type strain, carbon source peptone Aspergillus fumigatus
2.3.3.5 0.165
-
 complemented methylcitrate synthase mutant 11/12 B1, carbon source peptone Aspergillus fumigatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.5 acetyl-CoA + H2O + oxaloacetate
-
 Aspergillus fumigatus citrate + CoA
-
 ?
2.3.3.5 propionyl-CoA + H2O + oxaloacetate
-
 Aspergillus fumigatus (2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.3.5 Methylcitrate synthase
-
 Aspergillus fumigatus

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.3.5 CoA
-
 Aspergillus fumigatus

General Information

EC Number General Information Comment Organism
2.3.3.5 metabolism methylcitrate cycle Aspergillus fumigatus