EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.23 | 2-mercaptoethanol | the lower concentrations (1-10 mM) of 2-mercaptoethanol show a stimulating effect on activity | Lactiplantibacillus plantarum WCFS1 | |
3.2.1.23 | dithiothreitol | the lower concentrations (1-10 mM) of dithiothreitol show a stimulating effect on activity | Lactiplantibacillus plantarum WCFS1 | |
3.2.1.23 | Urea | the lower concentrations (1-10 mM) of urea show a stimulating effect on activity | Lactiplantibacillus plantarum WCFS1 |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.23 | beta-mercaptoethanol | 100 mM of beta-mercaptoethanol shows an inhibitory effect on activity (91% residual activity) | Lactiplantibacillus plantarum WCFS1 | |
3.2.1.23 | dithiothreitol | 100 mM of dithiothreitol shows an inhibitory effect on activity (84% residual activity) | Lactiplantibacillus plantarum WCFS1 | |
3.2.1.23 | EDTA | EDTA strongly inactivates the enzyme even at 1 mM (17% residual activity), 13% residual activity at 100 mM EDTA | Lactiplantibacillus plantarum WCFS1 | |
3.2.1.23 | Urea | 100 mM of urea shows an inhibitory effect on activity (48% residual activity) | Lactiplantibacillus plantarum WCFS1 |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.23 | 0.9 | - |
2-nitrophenyl beta-D-galactopyranoside | at pH 7.5 and 55°C | Lactiplantibacillus plantarum WCFS1 | |
3.2.1.23 | 29 | - |
lactose | at pH 7.0 and 60°C | Lactiplantibacillus plantarum WCFS1 |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.23 | K+ | 326% relative activity at 10 mM | Lactiplantibacillus plantarum WCFS1 | |
3.2.1.23 | Mg2+ | the enzyme needs MgCl2 for its stability retaining 73% and 85% of its activity after an incubation at 37°C for 5 h in the presence of 1 and 10 mM MgCl2, 414% relative activity at 10 mM | Lactiplantibacillus plantarum WCFS1 | |
3.2.1.23 | Mn2+ | 341% relative activity at 10 mM | Lactiplantibacillus plantarum WCFS1 | |
3.2.1.23 | Na+ | 165% relative activity at 10 mM | Lactiplantibacillus plantarum WCFS1 |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.23 | 35000 | - |
1 * 72000 + 1 * 35000, SDS-PAGE | Lactiplantibacillus plantarum WCFS1 |
3.2.1.23 | 35223 | - |
1 * 72180 + 1 * 35223, calculated from amino acid sequence | Lactiplantibacillus plantarum WCFS1 |
3.2.1.23 | 72000 | - |
1 * 72000 + 1 * 35000, SDS-PAGE | Lactiplantibacillus plantarum WCFS1 |
3.2.1.23 | 72180 | - |
1 * 72180 + 1 * 35223, calculated from amino acid sequence | Lactiplantibacillus plantarum WCFS1 |
3.2.1.23 | 107000 | - |
gel filtration | Lactiplantibacillus plantarum WCFS1 |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.23 | Lactiplantibacillus plantarum WCFS1 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.23 | p-aminobenzyl 1-thio-beta-D-galactopyranoside column chromatography | Lactiplantibacillus plantarum WCFS1 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.23 | 2-nitrophenyl beta-D-galactopyranoside + H2O | preferred substrate | Lactiplantibacillus plantarum WCFS1 | 2-nitrophenol + beta-D-galactose | - |
? | |
3.2.1.23 | lactose + H2O | preferred substrate | Lactiplantibacillus plantarum WCFS1 | D-galactose + D-glucose | - |
? | |
3.2.1.23 | additional information | the enzyme shows a strong preference for the formation of beta-(1-6) linkages in its transgalactosylation mode, while beta-(1-3)-linked products are formed to a lesser extent, comprising about 80% and 9%, respectively, of the newly formed glycosidic linkages in the oligosaccharide mixture at maximum galacto-oligosaccharides formation | Lactiplantibacillus plantarum WCFS1 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.23 | heterodimer | 1 * 72000 + 1 * 35000, SDS-PAGE | Lactiplantibacillus plantarum WCFS1 |
3.2.1.23 | heterodimer | 1 * 72180 + 1 * 35223, calculated from amino acid sequence | Lactiplantibacillus plantarum WCFS1 |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.23 | beta-D-galactoside galactohydrolase | - |
Lactiplantibacillus plantarum WCFS1 |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.23 | 55 | - |
for hydrolysis of 2-nitrophenyl beta-D-galactopyranoside | Lactiplantibacillus plantarum WCFS1 |
3.2.1.23 | 60 | - |
for hydrolysis of lactose | Lactiplantibacillus plantarum WCFS1 |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.23 | 92 | - |
2-nitrophenyl beta-D-galactopyranoside | at pH 7.5 and 55°C | Lactiplantibacillus plantarum WCFS1 | |
3.2.1.23 | 98 | - |
lactose | at pH 7.0 and 60°C | Lactiplantibacillus plantarum WCFS1 |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.23 | 7 | - |
for hydrolysis of lactose | Lactiplantibacillus plantarum WCFS1 |
3.2.1.23 | 7.5 | - |
for hydrolysis of 2-nitrophenyl beta-D-galactopyranoside | Lactiplantibacillus plantarum WCFS1 |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.23 | 6.5 | 8 | - |
Lactiplantibacillus plantarum WCFS1 |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.23 | 3.3 | - |
lactose | at pH 7.0 and 60°C | Lactiplantibacillus plantarum WCFS1 | |
3.2.1.23 | 130 | - |
2-nitrophenyl beta-D-galactopyranoside | at pH 7.5 and 55°C | Lactiplantibacillus plantarum WCFS1 |