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Literature summary extracted from

  • Iqbal, S.; Nguyen, T.H.; Nguyen, T.T.; Maischberger, T.; Haltrich, D.
    beta-Galactosidase from Lactobacillus plantarum WCFS1: biochemical characterization and formation of prebiotic galacto-oligosaccharides (2010), Carbohydr. Res., 345, 1408-1416.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.23 2-mercaptoethanol the lower concentrations (1-10 mM) of 2-mercaptoethanol show a stimulating effect on activity Lactiplantibacillus plantarum WCFS1
3.2.1.23 dithiothreitol the lower concentrations (1-10 mM) of dithiothreitol show a stimulating effect on activity Lactiplantibacillus plantarum WCFS1
3.2.1.23 Urea the lower concentrations (1-10 mM) of urea show a stimulating effect on activity Lactiplantibacillus plantarum WCFS1

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.23 beta-mercaptoethanol 100 mM of beta-mercaptoethanol shows an inhibitory effect on activity (91% residual activity) Lactiplantibacillus plantarum WCFS1
3.2.1.23 dithiothreitol 100 mM of dithiothreitol shows an inhibitory effect on activity (84% residual activity) Lactiplantibacillus plantarum WCFS1
3.2.1.23 EDTA EDTA strongly inactivates the enzyme even at 1 mM (17% residual activity), 13% residual activity at 100 mM EDTA Lactiplantibacillus plantarum WCFS1
3.2.1.23 Urea 100 mM of urea shows an inhibitory effect on activity (48% residual activity) Lactiplantibacillus plantarum WCFS1

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.23 0.9
-
 2-nitrophenyl beta-D-galactopyranoside at pH 7.5 and 55°C Lactiplantibacillus plantarum WCFS1
3.2.1.23 29
-
 lactose at pH 7.0 and 60°C Lactiplantibacillus plantarum WCFS1

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.23 K+ 326% relative activity at 10 mM Lactiplantibacillus plantarum WCFS1
3.2.1.23 Mg2+ the enzyme needs MgCl2 for its stability retaining 73% and 85% of its activity after an incubation at 37°C for 5 h in the presence of 1 and 10 mM MgCl2, 414% relative activity at 10 mM Lactiplantibacillus plantarum WCFS1
3.2.1.23 Mn2+ 341% relative activity at 10 mM Lactiplantibacillus plantarum WCFS1
3.2.1.23 Na+ 165% relative activity at 10 mM Lactiplantibacillus plantarum WCFS1

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.23 35000
-
 1 * 72000 + 1 * 35000, SDS-PAGE Lactiplantibacillus plantarum WCFS1
3.2.1.23 35223
-
 1 * 72180 + 1 * 35223, calculated from amino acid sequence Lactiplantibacillus plantarum WCFS1
3.2.1.23 72000
-
 1 * 72000 + 1 * 35000, SDS-PAGE Lactiplantibacillus plantarum WCFS1
3.2.1.23 72180
-
 1 * 72180 + 1 * 35223, calculated from amino acid sequence Lactiplantibacillus plantarum WCFS1
3.2.1.23 107000
-
 gel filtration Lactiplantibacillus plantarum WCFS1

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Lactiplantibacillus plantarum WCFS1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.23 p-aminobenzyl 1-thio-beta-D-galactopyranoside column chromatography Lactiplantibacillus plantarum WCFS1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O preferred substrate Lactiplantibacillus plantarum WCFS1 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 lactose + H2O preferred substrate Lactiplantibacillus plantarum WCFS1 D-galactose + D-glucose
-
 ?
3.2.1.23 additional information the enzyme shows a strong preference for the formation of beta-(1-6) linkages in its transgalactosylation mode, while beta-(1-3)-linked products are formed to a lesser extent, comprising about 80% and 9%, respectively, of the newly formed glycosidic linkages in the oligosaccharide mixture at maximum galacto-oligosaccharides formation Lactiplantibacillus plantarum WCFS1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.23 heterodimer 1 * 72000 + 1 * 35000, SDS-PAGE Lactiplantibacillus plantarum WCFS1
3.2.1.23 heterodimer 1 * 72180 + 1 * 35223, calculated from amino acid sequence Lactiplantibacillus plantarum WCFS1

Synonyms

EC Number Synonyms Comment Organism
3.2.1.23 beta-D-galactoside galactohydrolase
-
 Lactiplantibacillus plantarum WCFS1

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.23 55
-
 for hydrolysis of 2-nitrophenyl beta-D-galactopyranoside Lactiplantibacillus plantarum WCFS1
3.2.1.23 60
-
 for hydrolysis of lactose Lactiplantibacillus plantarum WCFS1

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.23 92
-
 2-nitrophenyl beta-D-galactopyranoside at pH 7.5 and 55°C Lactiplantibacillus plantarum WCFS1
3.2.1.23 98
-
 lactose at pH 7.0 and 60°C Lactiplantibacillus plantarum WCFS1

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 7
-
 for hydrolysis of lactose Lactiplantibacillus plantarum WCFS1
3.2.1.23 7.5
-
 for hydrolysis of 2-nitrophenyl beta-D-galactopyranoside Lactiplantibacillus plantarum WCFS1

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.23 6.5 8
-
 Lactiplantibacillus plantarum WCFS1

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.23 3.3
-
 lactose at pH 7.0 and 60°C Lactiplantibacillus plantarum WCFS1
3.2.1.23 130
-
 2-nitrophenyl beta-D-galactopyranoside at pH 7.5 and 55°C Lactiplantibacillus plantarum WCFS1