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Literature summary extracted from

  • Costa, H.; del Canto, S.; Ferrarotti, S.; de Jimenez Bonino, M.B.
    Structure-function relationship in cyclodextrin glycosyltransferase from Bacillus circulans DF 9R (2009), Carbohydr. Res., 344, 74-79.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.1.19 synthesis CGTases is used to catalyze cyclomaltooligosaccharides/cyclodextrins production in important industrial processes Niallia circulans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.19 expression of the enzyme in Escherichia coli strain DH5alpha Niallia circulans

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.19 Niallia circulans C4MH58
-
-
2.4.1.19 Niallia circulans DF 9R C4MH58
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.19 recombinant enzyme from Escherichia coli strain DH5alpha Niallia circulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.19 additional information CGTase catalyzes the formation of cyclomaltooligosaccharides, cyclic molecules formed by alpha-(1,4)-linked D-glucopyranosyl units with an apolar central cavity and a hydrophilic outer surface. alpha-, beta-Cyclizing and amylolytic activities withpotato starch as substrate, enzyme structure-function relationship, overview Niallia circulans ?
-
 ?
2.4.1.19 additional information CGTase catalyzes the formation of cyclomaltooligosaccharides, cyclic molecules formed by alpha-(1,4)-linked D-glucopyranosyl units with an apolar central cavity and a hydrophilic outer surface. alpha-, beta-Cyclizing and amylolytic activities withpotato starch as substrate, enzyme structure-function relationship, overview Niallia circulans DF 9R ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.19 More enzyme peptide mapping and sequencing, overview. Enzyme structure-function relationship, involving protein ethoxyformylation, overview Niallia circulans

Synonyms

EC Number Synonyms Comment Organism
2.4.1.19 CGTase
-
 Niallia circulans
2.4.1.19 cyclodextrin glycosyltransferase
-
 Niallia circulans
2.4.1.19 More the enzyme is a member of the GH13 family, also known as the alpha-amylase family1 Niallia circulans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.19 40
-
 amylolytic and alpha-cyclizing activity assay at Niallia circulans
2.4.1.19 55
-
 beta-cyclizing activity assay at Niallia circulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.19 5.5
-
 amylolytic activity assay at Niallia circulans
2.4.1.19 7
-
 alpha- and beta-cyclizing activity assay at Niallia circulans