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Literature summary extracted from

  • Cote, G.L.; Dunlap, C.A.; Vermillion, K.E.
    Glucosylation of raffinose via alternansucrase acceptor reactions (2009), Carbohydr. Res., 344, 1951-1959.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.1.140 drug development formation of oligosaccharides by alternansucrase may serve as prebiotics Leuconostoc mesenteroides

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.140 Leuconostoc mesenteroides
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 NRRL B-21297
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2.4.1.140 Leuconostoc mesenteroides NRRL B-21297
-
 NRRL B-21297
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.140 sucrose + raffinose the alternansucrase-catalyzed acceptor reaction with raffinose gives a gravimetric yield of alternan of 9.5% relative to the weight of sucrose, indicating that 20% of the glucosyl units are incorporated into alternan, and 80% into oligosaccharide acceptor products. The main products are the tetrasaccharides alpha-D-Glcp-(1-3)-alpha-D-Galp-(1-6)-alpha-D-Glcp-(1-2)-beta-D-Fruf and alpha-D-Glcp-(1-4)-alpha-D-Galp-(1-6)-alpha-D-Glcp-(1-2)-beta-D-Fruf in ratios ranging from 4:1 to 9:1, along with lesser amounts of alpha-D-Glcp-(1-6)-alpha-D-Galp-(1-6)-alpha-D-Glcp-(1-2)-beta-D-Fruf. Pentasaccharides, hexasaccharides and higher oligosaccharides are also produced Leuconostoc mesenteroides ?
-
 ?
2.4.1.140 sucrose + raffinose the alternansucrase-catalyzed acceptor reaction with raffinose gives a gravimetric yield of alternan of 9.5% relative to the weight of sucrose, indicating that 20% of the glucosyl units are incorporated into alternan, and 80% into oligosaccharide acceptor products. The main products are the tetrasaccharides alpha-D-Glcp-(1-3)-alpha-D-Galp-(1-6)-alpha-D-Glcp-(1-2)-beta-D-Fruf and alpha-D-Glcp-(1-4)-alpha-D-Galp-(1-6)-alpha-D-Glcp-(1-2)-beta-D-Fruf in ratios ranging from 4:1 to 9:1, along with lesser amounts of alpha-D-Glcp-(1-6)-alpha-D-Galp-(1-6)-alpha-D-Glcp-(1-2)-beta-D-Fruf. Pentasaccharides, hexasaccharides and higher oligosaccharides are also produced Leuconostoc mesenteroides NRRL B-21297 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.140 alternansucrase
-
 Leuconostoc mesenteroides
2.4.1.140 glucansucrase
-
 Leuconostoc mesenteroides