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Literature summary extracted from

  • Robyt, J.F.; Yoon, S.H.; Mukerjea, R.
    Dextransucrase and the mechanism for dextran biosynthesis (2008), Carbohydr. Res., 343, 3039-3048.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.5 expression of the truncated mutant enzyme, B-512FMC dextransucrase, in Escherichia coli Leuconostoc mesenteroides

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.5 additional information construction of a truncated mutant of enzyme B-512F, the mutant shows sigmoidal shaped curves when the initial velocities are plotted against the concentration of added dextran. The increase in the reaction rate and the decrease in the sigmoidal curve with increasing dextran concentrations indicate that dextran binds at a noncatalytic or allosteric site to give a more active enzyme Leuconostoc mesenteroides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.5 additional information
-
 additional information kinetic analysis of the low-molecular-weight products D-glucose, D-fructose, leucrose and isomaltodextrins formed during the synthesis of dextran by B-512FMC dextransucrase, overview Leuconostoc mesenteroides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.5 sucrose + [(1,6)-alpha-D-glucosyl]n Leuconostoc mesenteroides a highly processive mechanism of dextran biosynthesis, overview D-fructose + [(1,6)-alpha-D-glucosyl]n+1
-
 ?
2.4.1.5 sucrose + [(1,6)-alpha-D-glucosyl]n Leuconostoc mesenteroides B-512FMC a highly processive mechanism of dextran biosynthesis, overview D-fructose + [(1,6)-alpha-D-glucosyl]n+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.5 Leuconostoc mesenteroides
-
-
-
2.4.1.5 Leuconostoc mesenteroides B-512FMC
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.5 sucrose + [(1->6)-alpha-D-glucosyl]n = D-fructose + [(1->6)-alpha-D-glucosyl]n+1 involvement of the three conserved amino acids (Asp551, Glu589, and Asp662) in the organic reaction enzyme mechanism for the biosynthesis of dextran at the active site of dextransucrase by the insertion mechanism addition of D-glucose to the reducing-end of a growing dextran chain Leuconostoc mesenteroides
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.5 sucrose + isomaltose
-
 Leuconostoc mesenteroides D-fructose + isomaltotriose
-
 ?
2.4.1.5 sucrose + isomaltose
-
 Leuconostoc mesenteroides B-512FMC D-fructose + isomaltotriose
-
 ?
2.4.1.5 sucrose + [(1,6)-alpha-D-glucosyl]n a highly processive mechanism of dextran biosynthesis, overview Leuconostoc mesenteroides D-fructose + [(1,6)-alpha-D-glucosyl]n+1
-
 ?
2.4.1.5 sucrose + [(1,6)-alpha-D-glucosyl]n the enzyme performs dextran synthesis from sucrose, but D-glucose and sucrose are no initiator primers. Both D-glucose and dextran are covalently attached to B-512FMC dextransucrase at the active site during polymerization. The D-glucose moieties of sucrose are added to the reducing ends of the covalently linked growing dextran chains. Asp551, Glu589, and Asp 622 at the active sites of glucansucrases participate in the polymerization of dextran and related glucans from a single active site by the addition of the D-glucose moiety of sucrose to the reducing ends of the covalently linked glucan chains in a two catalytic-site, insertion mechanism overview. In the early stages of the reaction the products are D-glucose, D-fructose, leucrose, and isomaltodextrins in low, exponentially decreasing amounts from DP 2-5, with minuscule amounts of DP 6-12, but exponentially decreasing amounts of isomaltodextrins, down to minuscule amounts of DP 20-25, overview Leuconostoc mesenteroides D-fructose + [(1,6)-alpha-D-glucosyl]n+1
-
 ?
2.4.1.5 sucrose + [(1,6)-alpha-D-glucosyl]n a highly processive mechanism of dextran biosynthesis, overview Leuconostoc mesenteroides B-512FMC D-fructose + [(1,6)-alpha-D-glucosyl]n+1
-
 ?
2.4.1.5 sucrose + [(1,6)-alpha-D-glucosyl]n the enzyme performs dextran synthesis from sucrose, but D-glucose and sucrose are no initiator primers. Both D-glucose and dextran are covalently attached to B-512FMC dextransucrase at the active site during polymerization. The D-glucose moieties of sucrose are added to the reducing ends of the covalently linked growing dextran chains. Asp551, Glu589, and Asp 622 at the active sites of glucansucrases participate in the polymerization of dextran and related glucans from a single active site by the addition of the D-glucose moiety of sucrose to the reducing ends of the covalently linked glucan chains in a two catalytic-site, insertion mechanism overview. In the early stages of the reaction the products are D-glucose, D-fructose, leucrose, and isomaltodextrins in low, exponentially decreasing amounts from DP 2-5, with minuscule amounts of DP 6-12, but exponentially decreasing amounts of isomaltodextrins, down to minuscule amounts of DP 20-25, overview Leuconostoc mesenteroides B-512FMC D-fructose + [(1,6)-alpha-D-glucosyl]n+1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.5 B-512F dextransucrase
-
 Leuconostoc mesenteroides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.5 23
-
 assay at Leuconostoc mesenteroides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.5 5.2
-
-
 Leuconostoc mesenteroides