EC Number | Organism | UniProt | Comment | Textmining |
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2.4.1.264 | Xanthomonas campestris | - |
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EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.264 | UDP-glucuronate + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphoundecaprenol | structural analysis of the trisaccharide accumulating in mutatants with an insertion in the gumL gene | Xanthomonas campestris | UDP + GlcA-beta-(1-2)-D-Man-alpha-(1-3)-D-Glc-beta-(1-4)-D-Glc-alpha-1-diphosphoundecaprenol | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.264 | 20 | - |
assay at | Xanthomonas campestris |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
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2.4.1.264 | 8.2 | - |
assay at | Xanthomonas campestris |
EC Number | General Information | Comment | Organism |
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2.4.1.264 | malfunction | a transposon responsible for the mutation is located within gumK gene. The Tn5 insertion mutant with a reduced slimy phenotype fails to produce the pentasaccharide repeating-unit of xanthan. Only three sugars are transferred to the prenyl phosphate intermediate. The lipid-associated saccharide is the trisaccharide reducing end of the pentasaccharide from the wild-type strain. This trisaccharide is built up from UDP-Glc and GDP-Man, and a glucose residue is at the reducing end, linked to an allylic prenol through a diphosphate bridge. the trisaccharide-PP-polyprenol is the precursor of the polymer. This new polymer, a polytrisaccharide, is detected also in vivo. A recombinant plasmid with the whole gum cluster restored the wild type phenotype | Xanthomonas campestris |