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Literature summary extracted from

  • Matsumoto, K.; Yokote, H.; Arao, T.; Maegawa, M.; Tanaka, K.; Fujita, Y.; Shimizu, C.; Hanafusa, T.; Fujiwara, Y.; Nishio, K.
    N-Glycan fucosylation of epidermal growth factor receptor modulates receptor activity and sensitivity to epidermal growth factor receptor tyrosine kinase inhibitor (2008), Cancer Sci., 99, 1611-1617.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.68 additional information 293/EGFR/FUT8, HEK-293 cells transfected with epidermal growth factor receptor and FUT8, increased fucosylation of EGFR, about 20% increase of cellular growth in response to epidermal growth factor stiumlation, enhanced cellular sensitivity to gefitinib, decrease of epidermal growth factor-mediated phosphorylation of mitogen-activated protein kinase Homo sapiens
2.4.1.68 additional information 293/EGFR/shFUT8, stable knockdown mutant of intrinsic FUT8, about 20% decrease of cellular growth in response to epidermal growth factor stimulation Homo sapiens
2.4.1.68 additional information A549/FUT8, overexpression of FUT8 Homo sapiens
2.4.1.68 additional information A549/shFUT8, knockdown mutant of intrinsic FUT8, no enzyme activity, decrease of cellular growth in response to epidermal growth factor stimulation, decreased sensitivity of A549 cells to gefitinib Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.68 gefitinib
-
 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.68 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.68 A-549 cell
-
 Homo sapiens
-
2.4.1.68 HEK-293 cell
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.68 additional information
-
 44.1 U/l in HEK-293 cells transfected with epidermal growth factor receptor and FUT8, 1.8 U/l in HEK-293 cells transfected only with epidermal growth factor receptor Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.68 GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine-bi-(4-(2-pyridylamino)butylamine pH 7.0, 37°C, 6 h, reaction stopped by heating at 100°C for 1 min Homo sapiens GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-6)]-N-acetyl-beta-D-glucosaminyl]asparagine-bi-(4-(2-pyridylamino)butylamine)
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.68 alpha1,6-fucosyltransferase
-
 Homo sapiens
2.4.1.68 FUT8
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.68 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.68 7
-
 assay at Homo sapiens