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Literature summary extracted from

  • Zibaee, A.; Bandani, A.R.; Ramzi, S.
    Enzymatic properties of alpha- and beta-glucosidases extracted from midgut and salivary glands of rice striped stem borer, Chilo suppressalis Walker (Lepidoptera: Pyralidae) (2009), C. R. Biol., 332, 633-641.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.20 CaCl2 40 mmol/l, 225.53% relative enzyme activity in midgut, 190.24% relative enzyme activity in salivary glands Chilo suppressalis
3.2.1.20 MgCl2 40 mmol/l, 206.2% relative enzyme activity in midgut, 166% relative enzyme activity in salivary glands Chilo suppressalis
3.2.1.20 NaCl 40 mmol/l, 223.25% relative enzyme activity in midgut, 193.54% relative enzyme activity in salivary glands Chilo suppressalis
3.2.1.21 CaCl2 40 mmol/l, 233.33% relative enzyme activity in midgut, 193.31% relative enzyme activity in salivary glands Chilo suppressalis
3.2.1.21 MgCl2 40 mmol/l, 200% relative enzyme activity in midgut, 169.49% relative enzyme activity in salivary glands Chilo suppressalis
3.2.1.21 NaCl 40 mmol/l, 198.57% relative enzyme activity in midgut, 163.63% relative enzyme activity in salivary glands Chilo suppressalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.20 EDTA 4 mmol/l, 63.27% relative enzyme activity in midgut, 70% relative enzyme activity in salivary glands Chilo suppressalis
3.2.1.20 KCl 40 mmol/l, 36.04% relative enzyme activity in midgut, 29.26% relative enzyme activity in salivary glands Chilo suppressalis
3.2.1.20 SDS 8 mmol/l, 86.72% relative enzyme activity in midgut, 60.41% relative enzyme activity in salivary glands Chilo suppressalis
3.2.1.20 Urea 6000 mmol/l, 26.74% relative enzyme activity in midgut, 38.26% relative enzyme activity in salivary glands Chilo suppressalis
3.2.1.21 EDTA 46.49% residual activity of the enzyme from midgut at 4 mM EDTA, 29.8% residual activity of the enzyme from salivary gland at 4 mM EDTA; 4 mmol/l, 46.49% relative enzyme activity in midgut, 29.8% relative enzyme activity in salivary glands Chilo suppressalis
3.2.1.21 K+ 58.03% residual activity of the enzyme from midgut at 40 mM K+, 45.95% residual activity of the enzyme from salivary gland at 40 mM K+ Chilo suppressalis
3.2.1.21 KCl 40 mmol/l, 58.03% relative enzyme activity in midgut, 45.95% relative enzyme activity in salivary glands Chilo suppressalis
3.2.1.21 SDS 61.32% residual activity of the enzyme from midgut at 8 mM SDS, 43.75% residual activity of the enzyme from salivary gland at 8 mM SDS; 8 mmol/l, 61.32% relative enzyme activity in midgut, 43.75% relative enzyme activity in salivary glands Chilo suppressalis
3.2.1.21 Urea 53.8% residual activity of the enzyme from midgut at 4 M urea, 20.05% residual activity of the enzyme from salivary gland at 6 M urea; 6000 mmol/l, 56.41% relative enzyme activity in midgut, 20.05% relative enzyme activity in salivary glands Chilo suppressalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.20 1.24
-
 p-nitrophenyl-alpha-D-glucopyranoside salivary gland Chilo suppressalis
3.2.1.20 1.71
-
 p-nitrophenyl-alpha-D-glucopyranoside midgut Chilo suppressalis
3.2.1.21 0.39
-
 4-nitrophenyl-beta-D-glucopyranoside enzyme from salivary gland, in 100 mM universal buffer (pH 5.0) at 37°C Chilo suppressalis
3.2.1.21 0.61
-
 4-nitrophenyl-beta-D-glucopyranoside enzyme from midgut, in 100 mM universal buffer (pH 5.0) at 37°C Chilo suppressalis
3.2.1.21 0.61
-
 p-nitrophenyl-beta-D-glucopyranoside midgut Chilo suppressalis
3.2.1.21 3.39
-
 p-nitrophenyl-beta-D-glucopyranoside salivary glands Chilo suppressalis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.21 Ca2+ 233.33% relative activity of the enzyme from midgut at 40 mM Ca2+, 193.31% relative activity of the enzyme from salivary gland at 40 mM Ca2+ Chilo suppressalis
3.2.1.21 Mg2+ 200% relative activity of the enzyme from midgut at 40 mM Mg2+, 169.49% relative activity of the enzyme from salivary gland at 40 mM Mg2+ Chilo suppressalis
3.2.1.21 Na+ 198.57% relative activity of the enzyme from midgut at 40 mM Na+, 163.63% relative activity of the enzyme from salivary gland at 40 mM Na+ Chilo suppressalis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.20 Chilo suppressalis
-
-
-
3.2.1.21 Chilo suppressalis
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.20 larva
-
 Chilo suppressalis
-
3.2.1.20 midgut
-
 Chilo suppressalis
-
3.2.1.20 salivary gland
-
 Chilo suppressalis
-
3.2.1.21 larva
-
 Chilo suppressalis
-
3.2.1.21 midgut
-
 Chilo suppressalis
-
3.2.1.21 salivary gland
-
 Chilo suppressalis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.20 0.0000063
-
 in salivary gland Chilo suppressalis
3.2.1.20 0.000009
-
 in midgut Chilo suppressalis
3.2.1.21 0.000003
-
 in salivary gland Chilo suppressalis
3.2.1.21 0.000005
-
 in midgut Chilo suppressalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.20 4-nitrophenyl alpha-D-glucopyranoside + H2O assay at pH 5.0, 37°C, reaction stopped by adding sodium carbonate Chilo suppressalis 4-nitrophenol + D-glucose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Chilo suppressalis 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 p-nitrophenyl-beta-D-glucopyranoside + H2O assay at pH 5.0, 37°C, reaction stopped by adding sodium carbonate Chilo suppressalis p-nitrophenol + beta-D-glucose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.20 alpha-glucosidase
-
 Chilo suppressalis
3.2.1.21 beta-glucosidase
-
 Chilo suppressalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.20 45
-
-
 Chilo suppressalis
3.2.1.21 45
-
-
 Chilo suppressalis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 20 45 activity increases steadily from 20-45°C Chilo suppressalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.20 8
-
-
 Chilo suppressalis
3.2.1.21 8
-
-
 Chilo suppressalis
3.2.1.21 9
-
-
 Chilo suppressalis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.20 3 8 increase of enzyme activity with pH increase, above 8 decrease of enzyme activity with increasing pH Chilo suppressalis
3.2.1.21 3 8 increase of enzyme activity with pH increase, above 8 decrease of enzyme activity with increasing pH Chilo suppressalis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.20 3 8 increase of enzyme activity with pH increase, above 8 decrease of enzyme activity with increasing pH Chilo suppressalis
3.2.1.21 3 8 increase of enzyme activity with pH increase, above 8 decrease of enzyme activity with increasing pH Chilo suppressalis