EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.3.13 | the coding sequences of alpha-IPMS-2CR and alpha-IPMS-14CR are cloned into the vector pET15b for expression in Escherichia coli BL21DE3 cells | Mycobacterium tuberculosis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.3.13 | Y410F | mutant, insensitive to feedback inhibition | Mycobacterium tuberculosis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.3.13 | 0.027 | - |
acetyl-CoA | alpha-IPMS-14CR | Mycobacterium tuberculosis | |
2.3.3.13 | 0.035 | - |
alpha-ketoisovaleric acid | alpha-IPMS-14CR | Mycobacterium tuberculosis | |
2.3.3.13 | 0.261 | - |
alpha-ketoisovaleric acid | alpha-IPMS-2CR | Mycobacterium tuberculosis | |
2.3.3.13 | 0.568 | - |
acetyl-CoA | alpha-IPMS-2CR | Mycobacterium tuberculosis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.3.3.13 | 74000 | - |
alpha-IPMS-2CR, determined by SDS-PAGE | Mycobacterium tuberculosis |
2.3.3.13 | 99000 | - |
alpha-IPMS-14CR, determined by SDS-PAGE | Mycobacterium tuberculosis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.3.13 | acetyl-CoA + 3-methyl-2-oxobutanoate + H2O | Mycobacterium tuberculosis | - |
(2S)-2-isopropylmalate + CoA | - |
? | |
2.3.3.13 | acetyl-CoA + 3-methyl-2-oxobutanoate + H2O | Mycobacterium tuberculosis H37Rv | - |
(2S)-2-isopropylmalate + CoA | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.3.13 | Mycobacterium tuberculosis | P9WQB3 | - |
- |
2.3.3.13 | Mycobacterium tuberculosis H37Rv | P9WQB3 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.3.13 | using Ni-NTA resin | Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.3.13 | acetyl-CoA + 3-methyl-2-oxobutanoate + H2O | - |
Mycobacterium tuberculosis | (2S)-2-isopropylmalate + CoA | - |
? | |
2.3.3.13 | acetyl-CoA + 3-methyl-2-oxobutanoate + H2O | - |
Mycobacterium tuberculosis H37Rv | (2S)-2-isopropylmalate + CoA | - |
? | |
2.3.3.13 | alpha-ketoisovaleric acid + H2O + acetyl-CoA | - |
Mycobacterium tuberculosis | ? + CoA | - |
? | |
2.3.3.13 | alpha-ketoisovaleric acid + H2O + acetyl-CoA | - |
Mycobacterium tuberculosis H37Rv | ? + CoA | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.3.3.13 | homodimer | 2 * 74000, alpha-IPMS-2CR, 2 * 99000, alpha-IPMS-14CR, determined by gel filtration | Mycobacterium tuberculosis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.3.13 | alpha-IPMS | - |
Mycobacterium tuberculosis |
2.3.3.13 | alpha-IPMS-14CR | - |
Mycobacterium tuberculosis |
2.3.3.13 | alpha-IPMS-14CR | the gene encoding alpha-IPMS is polymorphic due to the insertion of 57 bp repeat units, alpha-IPMS-14CR comprises 14 copies | Mycobacterium tuberculosis |
2.3.3.13 | alpha-IPMS-2CR | the gene encoding alpha-IPMS is polymorphic due to the insertion of 57 bp repeat units, alpha-IPMS-2CR comprises two copies | Mycobacterium tuberculosis |
2.3.3.13 | Alpha-isopropylmalate synthase | - |
Mycobacterium tuberculosis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.3.13 | 37 | - |
activity assay | Mycobacterium tuberculosis |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.3.13 | 37 | 42 | - |
Mycobacterium tuberculosis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.3.13 | 0.52 | - |
alpha-ketoisovaleric acid | alpha-IPMS-14CR | Mycobacterium tuberculosis | |
2.3.3.13 | 0.61 | - |
acetyl-CoA | alpha-IPMS-14CR | Mycobacterium tuberculosis | |
2.3.3.13 | 1.17 | - |
alpha-ketoisovaleric acid | alpha-IPMS-2CR | Mycobacterium tuberculosis | |
2.3.3.13 | 2.22 | - |
acetyl-CoA | alpha-IPMS-2CR | Mycobacterium tuberculosis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.3.13 | 8.5 | - |
activity assay | Mycobacterium tuberculosis |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.3.3.13 | 7 | 8.5 | - |
Mycobacterium tuberculosis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.3.3.13 | acetyl-CoA | - |
Mycobacterium tuberculosis |