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Literature summary extracted from

  • Yindeeyoungyeon, W.; Likitvivatanavong, S.; Palittapongarnpim, P.
    Characterization of alpha-isopropylmalate synthases containing different copy numbers of tandem repeats in Mycobacterium tuberculosis (2009), BMC Microbiol., 9, 122.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.3.13 the coding sequences of alpha-IPMS-2CR and alpha-IPMS-14CR are cloned into the vector pET15b for expression in Escherichia coli BL21DE3 cells Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
2.3.3.13 Y410F mutant, insensitive to feedback inhibition Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.13 0.027
-
acetyl-CoA alpha-IPMS-14CR Mycobacterium tuberculosis
2.3.3.13 0.035
-
alpha-ketoisovaleric acid alpha-IPMS-14CR Mycobacterium tuberculosis
2.3.3.13 0.261
-
alpha-ketoisovaleric acid alpha-IPMS-2CR Mycobacterium tuberculosis
2.3.3.13 0.568
-
acetyl-CoA alpha-IPMS-2CR Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.13 74000
-
alpha-IPMS-2CR, determined by SDS-PAGE Mycobacterium tuberculosis
2.3.3.13 99000
-
alpha-IPMS-14CR, determined by SDS-PAGE Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.13 acetyl-CoA + 3-methyl-2-oxobutanoate + H2O Mycobacterium tuberculosis
-
(2S)-2-isopropylmalate + CoA
-
?
2.3.3.13 acetyl-CoA + 3-methyl-2-oxobutanoate + H2O Mycobacterium tuberculosis H37Rv
-
(2S)-2-isopropylmalate + CoA
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.13 Mycobacterium tuberculosis P9WQB3
-
-
2.3.3.13 Mycobacterium tuberculosis H37Rv P9WQB3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.13 using Ni-NTA resin Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.13 acetyl-CoA + 3-methyl-2-oxobutanoate + H2O
-
Mycobacterium tuberculosis (2S)-2-isopropylmalate + CoA
-
?
2.3.3.13 acetyl-CoA + 3-methyl-2-oxobutanoate + H2O
-
Mycobacterium tuberculosis H37Rv (2S)-2-isopropylmalate + CoA
-
?
2.3.3.13 alpha-ketoisovaleric acid + H2O + acetyl-CoA
-
Mycobacterium tuberculosis ? + CoA
-
?
2.3.3.13 alpha-ketoisovaleric acid + H2O + acetyl-CoA
-
Mycobacterium tuberculosis H37Rv ? + CoA
-
?

Subunits

EC Number Subunits Comment Organism
2.3.3.13 homodimer 2 * 74000, alpha-IPMS-2CR, 2 * 99000, alpha-IPMS-14CR, determined by gel filtration Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.3.3.13 alpha-IPMS
-
Mycobacterium tuberculosis
2.3.3.13 alpha-IPMS-14CR
-
Mycobacterium tuberculosis
2.3.3.13 alpha-IPMS-14CR the gene encoding alpha-IPMS is polymorphic due to the insertion of 57 bp repeat units, alpha-IPMS-14CR comprises 14 copies Mycobacterium tuberculosis
2.3.3.13 alpha-IPMS-2CR the gene encoding alpha-IPMS is polymorphic due to the insertion of 57 bp repeat units, alpha-IPMS-2CR comprises two copies Mycobacterium tuberculosis
2.3.3.13 Alpha-isopropylmalate synthase
-
Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.3.13 37
-
activity assay Mycobacterium tuberculosis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.3.3.13 37 42
-
Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.3.13 0.52
-
alpha-ketoisovaleric acid alpha-IPMS-14CR Mycobacterium tuberculosis
2.3.3.13 0.61
-
acetyl-CoA alpha-IPMS-14CR Mycobacterium tuberculosis
2.3.3.13 1.17
-
alpha-ketoisovaleric acid alpha-IPMS-2CR Mycobacterium tuberculosis
2.3.3.13 2.22
-
acetyl-CoA alpha-IPMS-2CR Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.3.13 8.5
-
activity assay Mycobacterium tuberculosis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.3.13 7 8.5
-
Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.3.13 acetyl-CoA
-
Mycobacterium tuberculosis