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Literature summary extracted from

  • Ogata, M.; Nakajima, M.; Kato, T.; Obara, T.; Yagi, H.; Kato, K.; Usui, T.; Park, E.Y.
    Synthesis of sialoglycopolypeptide for potentially blocking influenza virus infection using a rat alpha2,6-sialyltransferase expressed in BmNPV bacmid-injected silkworm larvae (2009), BMC Biotechnol., 9, 54.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.3.1 additional information in the sialyltransferase activity assay, FLAG-tagged ST6Gal1 has the highest expression level, which is 1.8- or 4.1fold higher than that of Strep- or His-tagged ST6Gal1, respectively Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.3.1 ST6Gal1 cloned as N-terminal His-, Strep- and FLAG-tagged fusion proteins. FLAG-tagged recombinant ST6Gal1 expressed in fifth instar silkworm larval hemolymph using recombinant both cysteine protease- and chitinase-deficient Bombyx mori nucleopolyhedrovirus (BmNPV-CP--Chi-) bacmid Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.3.1 0.92
-
 5-(5-dimethylaminonaphthalene-1-sulfonyl-2-(2-aminoethoxy))ethyl beta-N-acetyllactosamine
-
 Rattus norvegicus
2.4.3.1 2.8
-
 5-(5-dimethylaminonaphthalene-1-sulfonyl-2-(2-aminoethoxy))ethyl beta-lactose
-
 Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.3.1 MnCl2
-
 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.3.1 40000
-
 1 * 40000, SDS-PAGE Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.4.3.1 Rattus norvegicus P13721
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.3.1 FLAG-tagged recombinant ST6Gal1 purified by precipitation with ammonium sulphate followed by affinity chromatography, with a recovery yield of 64%, 159fold Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.3.1 0.016
-
 crude enzyme Rattus norvegicus
2.4.3.1 2.6
-
 159fold purified enzyme Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.3.1 CMP-N-acetylneuraminate + 5-(5-dimethylaminonaphthalene-1-sulfonyl-2-(2-aminoethoxy))ethyl beta-lactose
-
 Rattus norvegicus CMP + ?
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + 5-(5-dimethylaminonaphthalene-1-sulfonyl-2-(2-aminoethoxy))ethyl beta-N-acetyllactosamine
-
 Rattus norvegicus CMP + 5-(5-dimethylaminonaphthalene-1-sulfonyl-2-(2-aminoethoxy)) ethyl beta-N-acetylneuraminic acid alpha-2,6-N-acetyllactosamine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.3.1 monomer 1 * 40000, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
2.4.3.1 alpha2,6-sialyltransferase
-
 Rattus norvegicus
2.4.3.1 ST6Gal1
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.3.1 37
-
 purified enzyme Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.3.1 20
-
 purified enzyme is stable below 20°C but is rapidly inactivated at temperatures above 20°C Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.3.1 0.087
-
 5-(5-dimethylaminonaphthalene-1-sulfonyl-2-(2-aminoethoxy))ethyl beta-lactose
-
 Rattus norvegicus
2.4.3.1 1.13
-
 5-(5-dimethylaminonaphthalene-1-sulfonyl-2-(2-aminoethoxy))ethyl beta-N-acetyllactosamine
-
 Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.3.1 6
-
 purified enzyme Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.3.1 5 9 purified enzyme is relatively stable over a wide range of pH Rattus norvegicus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.3.1 Rattus norvegicus isoelectric focusing
-
 6.3

General Information

EC Number General Information Comment Organism
2.4.3.1 physiological function recombinant ST6Gal1 can be used as a key enzyme for the synthesis of an artificial sialoglycopolymer for potentially blocking influenza virus infection Rattus norvegicus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.3.1 0.031
-
 5-(5-dimethylaminonaphthalene-1-sulfonyl-2-(2-aminoethoxy))ethyl beta-lactose
-
 Rattus norvegicus
2.4.3.1 1.23
-
 5-(5-dimethylaminonaphthalene-1-sulfonyl-2-(2-aminoethoxy))ethyl beta-N-acetyllactosamine
-
 Rattus norvegicus