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Literature summary extracted from

  • Mueller, M.; Nidetzky, B.
    Orthophosphate binding at the dimer interface of Corynebacterium callunae starch phosphorylase: mutational analysis of its role for activity and stability of the enzyme (2010), BMC Biochem., 11, 008.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.1 L31A mutation decreases the strong protective effect of phosphate at 10 or 100 mM against inactivation at 45°C and subunit dissociation enforced by imidazole. Loss of stability appears to be largely due to weakened affinity for orthophosphate binding Corynebacterium callunae
2.4.1.1 R141A mutation disrupts the strong protective effect of phosphate at 10 or 100 mM against inactivation at 45°C and subunit dissociation enforced by imidazole. Loss of stability appears to be largely due to weakened affinity for orthophosphate binding Corynebacterium callunae
2.4.1.1 S174A mutation decreases the strong protective effect of phosphate at 10 or 100 mM against inactivation at 45°C and subunit dissociation enforced by imidazole. Loss of stability appears to be largely due to weakened affinity for orthophosphate binding Corynebacterium callunae
2.4.1.1 T28A mutation disrupts the strong protective effect of phosphate at 10 or 100 mM against inactivation at 45°C and subunit dissociation enforced by imidazole. Loss of stability appears to be largely due to weakened affinity for orthophosphate binding Corynebacterium callunae

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.1 Corynebacterium callunae Q8KQ56
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.1 27
-
30°C, pH 7.0, mutant R141A Corynebacterium callunae
2.4.1.1 29
-
30°C, pH 7.0, mutant K31A Corynebacterium callunae
2.4.1.1 29
-
30°C, pH 7.0, mutant S174A Corynebacterium callunae
2.4.1.1 29
-
30°C, pH 7.0, mutant T28A Corynebacterium callunae
2.4.1.1 31
-
30°C, pH 7.0, wild-type Corynebacterium callunae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.1 maltodextrin + phosphate
-
Corynebacterium callunae ? + alpha-D-glucose 1-phosphate
-
r

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.1 45
-
half-life 2.6 min for wild-type, 0.8 min for mutant T28A, 5.3 min for mutant K31A, 2.8 min for mutant S174A, 2.9 min for mutant R141A. In presence of 10 mM phosphate, half-life 280 min for wild-type, 2.2 min for mutant T28A, 170 min for mutant K31A, 33 min for mutant S174A, 7.5 min for mutant R141A. In presence of 100 mM phosphate, half-life 3500 min for wild-type, 330 min for mutant T28A, 3500 min for mutant K31A, 990 min for mutant S174A, 1400 min for mutant R141A Corynebacterium callunae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.1 40
-
phosphate 30°C, pH 7.0, mutant R141A Corynebacterium callunae
2.4.1.1 44
-
phosphate 30°C, pH 7.0, mutant K31A Corynebacterium callunae
2.4.1.1 44
-
phosphate 30°C, pH 7.0, mutant S174A Corynebacterium callunae
2.4.1.1 44
-
phosphate 30°C, pH 7.0, mutant T28A Corynebacterium callunae
2.4.1.1 47
-
phosphate 30°C, pH 7.0, wild-type Corynebacterium callunae

Cofactor

EC Number Cofactor Comment Organism Structure
2.4.1.1 pyridoxal 5'-phosphate about 0.7 mol per mol of protein Corynebacterium callunae