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Literature summary extracted from

  • Tran, S.T.; Le, D.T.; Kim, Y.C.; Shin, M.; Choi, J.D.
    Cloning and characterization of phosphomannose isomerase from Sphingomonas chungbukensis DJ77 (2009), BMB Rep., 42, 523-528.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.8 gene pmi, DNA and amino acid sequence determination and analysis, expression of the His6-tagged enzyme in Escherichia coli strain BL21 (DE3) Sphingobium chungbukense

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.8 GDP-D-mannose inhibits mannose 6-phosphate isomerase activity of PMI, feedback regulation of this pathway Sphingobium chungbukense
5.3.1.8 additional information no inhibition by GTP, mannose 1-phosphate, and phosphodiphosphate Sphingobium chungbukense

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.8 Ca2+ activates Sphingobium chungbukense
5.3.1.8 Co2+ beste metal ion activator Sphingobium chungbukense
5.3.1.8 Mg2+ activates only the GMP activity of the enzyme Sphingobium chungbukense
5.3.1.8 additional information PMI absolutely requires divalent metal cations for catalytic activity Sphingobium chungbukense
5.3.1.8 Ni2+ activates Sphingobium chungbukense
5.3.1.8 Zn2+ activates Sphingobium chungbukense

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.8 50000
-
 x * 50000, recombinant His6-tagged enzyme, SDS-PAGE Sphingobium chungbukense

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.1.8 D-Mannose 6-phosphate Sphingobium chungbukense
-
 D-Fructose 6-phosphate
-
 r
5.3.1.8 D-Mannose 6-phosphate Sphingobium chungbukense DJ77
-
 D-Fructose 6-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.8 Sphingobium chungbukense
-
 gene pmi
-
5.3.1.8 Sphingobium chungbukense DJ77
-
 gene pmi
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.8 recombinant His6-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography Sphingobium chungbukense

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.8 D-Mannose 6-phosphate
-
 Sphingobium chungbukense D-Fructose 6-phosphate
-
 r
5.3.1.8 D-Mannose 6-phosphate
-
 Sphingobium chungbukense DJ77 D-Fructose 6-phosphate
-
 r
5.3.1.8 additional information PMI is bifunctional possessing both mannose 6-phosphate isomerase and GDP-mannose diphosphorylase activities Sphingobium chungbukense ?
-
 ?
5.3.1.8 additional information PMI is bifunctional possessing both mannose 6-phosphate isomerase and GDP-mannose diphosphorylase activities Sphingobium chungbukense DJ77 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.1.8 ? x * 50000, recombinant His6-tagged enzyme, SDS-PAGE Sphingobium chungbukense
5.3.1.8 More PMI possesses two domains with three conserved motifs: a GMP domain at the N-terminus and a PMI domain at the C-terminus Sphingobium chungbukense

Synonyms

EC Number Synonyms Comment Organism
5.3.1.8 Phosphomannose isomerase
-
 Sphingobium chungbukense
5.3.1.8 PMI
-
 Sphingobium chungbukense
5.3.1.8 PMI/GMP
-
 Sphingobium chungbukense

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.8 37
-
 assay at Sphingobium chungbukense

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.8 8
-
-
 Sphingobium chungbukense

General Information

EC Number General Information Comment Organism
5.3.1.8 metabolism phosphomannose isomerase catalyzes the interconversion of fructose-6-phosphate and mannose-6-phosphate in the extracellular polysaccharide synthesis pathway, feedback regulation of this pathway Sphingobium chungbukense