Any feedback?
Literature summary extracted from
- High-level expression, purification and characterization of a recombinant medium-temperature alpha-amylase from Bacillus subtilis (2010), Biotechnol. Lett., 32, 119-124.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.1 | expressed in Bacillus subtilis strain WB600 | Bacillus subtilis |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 48000 | - |
SDS-PAGE | Bacillus subtilis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.1 | soluble starch + H2O | Bacillus subtilis | - |
malto-oligosaccharides | - |
? |  |
| 3.2.1.1 | soluble starch + H2O | Bacillus subtilis BF768 | - |
malto-oligosaccharides | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.1 | Bacillus subtilis | B8Y1H0 | - |
- |
| 3.2.1.1 | Bacillus subtilis BF768 | B8Y1H0 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.1 | ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Sephadex G-75gel filtration | Bacillus subtilis |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | additional information | - |
the recombinant enzyme shows a specific activity of 21.3 units/mg in the extract supernatant and of 35.8 units/mg after 1.7fold purification, one unit is defined as the amount of enzyme required to liquefy 1 g soluble starch completely for 1 h at 60°C and pH 6.0 | Bacillus subtilis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.1 | soluble starch + H2O | - |
Bacillus subtilis | malto-oligosaccharides | - |
? | |
| 3.2.1.1 | soluble starch + H2O | - |
Bacillus subtilis BF768 | malto-oligosaccharides | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.1 | alpha-1,4-glucan-4-glucanohydrolase | - |
Bacillus subtilis |
| 3.2.1.1 | AmyE | - |
Bacillus subtilis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 60 | - |
purified recombinant enzyme | Bacillus subtilis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 40 | 80 | after incubation at 40, 60 and 80°C for 1 h, the recombinant enzyme retains nearly 90, 80 and 50% of its initial activity, respectively. 83% of its initial activity is lost after being treated at 100°C for 20 min | Bacillus subtilis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 6 | - |
purified recombinant enzyme | Bacillus subtilis |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 5.5 | 7.5 | - |
Bacillus subtilis |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
