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Literature summary extracted from

  • Wang, X.D.; Zhang, X.E.; Guo, Y.C.; Zhang, Z.P.; Cao, Z.A.; Zhou, Y.F.
    Characterization of glycerol dehydratase expressed by fusing its alpha- and beta-subunits (2009), Biotechnol. Lett., 31, 711-717.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.30 adenosylcobalamin
-
Klebsiella pneumoniae
4.2.1.30 ATP
-
Klebsiella pneumoniae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.30 PCR products of gdh genes for co-expressing alpha-, beta- and gamma subunits of wild-type GDH or GDHA/CB and GDHAB/C fusion constructs inserted into pET32a vector using NdeI/HindIII sites. To minimize the effect of steric hindrance, a linker peptide inserted into the fusion partners by SacI sites, resulting two plasmids (pET32aGDHA/CLB and pET32aGDHALB/C) for expressing fusion enzyme GDHA/CLB and GDHALB/C, expressed in Escherichia coli AD494(DE3) Klebsiella pneumoniae

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.30 glycerol similar to the wild-type enzyme, GDHAB/C and GDHALB/C undergo mechanism-based inactivation by glycerol during catalysis and can be reactivated by glycerol dehydratase reactivase (GDHR), adenosylcobalamin, ATP, and MgCl2 Klebsiella pneumoniae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.30 Mg2+
-
Klebsiella pneumoniae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.30 17700
-
x * 60700 + x * 229000 + x * 161000, alphabetagamma including His6-tag, SDS-PAGE. x * 83800 + x * 17700, GDHAB/C, SDS-PAGE. x * 85100 + x * 17700, GDHALB/C, SDS-PAGE. x * 60700 + x * 40900, GDHA/CB. x * 60700 + x * 42200, GDHA/CLB, SDS-PAGE Klebsiella pneumoniae
4.2.1.30 40900
-
x * 60700 + x * 229000 + x * 161000, alphabetagamma including His6-tag, SDS-PAGE. x * 83800 + x * 17700, GDHAB/C, SDS-PAGE. x * 85100 + x * 17700, GDHALB/C, SDS-PAGE. x * 60700 + x * 40900, GDHA/CB. x * 60700 + x * 42200, GDHA/CLB, SDS-PAGE Klebsiella pneumoniae
4.2.1.30 42200
-
x * 60700 + x * 229000 + x * 161000, alphabetagamma including His6-tag, SDS-PAGE. x * 83800 + x * 17700, GDHAB/C, SDS-PAGE. x * 85100 + x * 17700, GDHALB/C, SDS-PAGE. x * 60700 + x * 40900, GDHA/CB. x * 60700 + x * 42200, GDHA/CLB, SDS-PAGE Klebsiella pneumoniae
4.2.1.30 60700
-
x * 60700 + x * 229000 + x * 161000, alphabetagamma including His6-tag, SDS-PAGE. x * 83800 + x * 17700, GDHAB/C, SDS-PAGE. x * 85100 + x * 17700, GDHALB/C, SDS-PAGE. x * 60700 + x * 40900, GDHA/CB. x * 60700 + x * 42200, GDHA/CLB, SDS-PAGE Klebsiella pneumoniae
4.2.1.30 83800
-
x * 60700 + x * 229000 + x * 161000, alphabetagamma including His6-tag, SDS-PAGE. x * 83800 + x * 17700, GDHAB/C, SDS-PAGE. x * 85100 + x * 17700, GDHALB/C, SDS-PAGE. x * 60700 + x * 40900, GDHA/CB. x * 60700 + x * 42200, GDHA/CLB, SDS-PAGE Klebsiella pneumoniae
4.2.1.30 85100
-
x * 60700 + x * 229000 + x * 161000, alphabetagamma including His6-tag, SDS-PAGE. x * 83800 + x * 17700, GDHAB/C, SDS-PAGE. x * 85100 + x * 17700, GDHALB/C, SDS-PAGE. x * 60700 + x * 40900, GDHA/CB. x * 60700 + x * 42200, GDHA/CLB, SDS-PAGE Klebsiella pneumoniae
4.2.1.30 161000
-
x * 60700 + x * 229000 + x * 161000, alphabetagamma including His6-tag, SDS-PAGE. x * 83800 + x * 17700, GDHAB/C, SDS-PAGE. x * 85100 + x * 17700, GDHALB/C, SDS-PAGE. x * 60700 + x * 40900, GDHA/CB. x * 60700 + x * 42200, GDHA/CLB, SDS-PAGE Klebsiella pneumoniae
4.2.1.30 229000
-
x * 60700 + x * 229000 + x * 161000, alphabetagamma including His6-tag, SDS-PAGE. x * 83800 + x * 17700, GDHAB/C, SDS-PAGE. x * 85100 + x * 17700, GDHALB/C, SDS-PAGE. x * 60700 + x * 40900, GDHA/CB. x * 60700 + x * 42200, GDHA/CLB, SDS-PAGE Klebsiella pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.30 Klebsiella pneumoniae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.30 by either ion exchange chromatography or on nickel chelate column Klebsiella pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.30 1,2-propanediol
-
Klebsiella pneumoniae propionaldehyde + H2O
-
?
4.2.1.30 glycerol
-
Klebsiella pneumoniae 3-hydroxypropanal + H2O
-
?

Subunits

EC Number Subunits Comment Organism
4.2.1.30 ? x * 60700 + x * 229000 + x * 161000, alphabetagamma including His6-tag, SDS-PAGE. x * 83800 + x * 17700, GDHAB/C, SDS-PAGE. x * 85100 + x * 17700, GDHALB/C, SDS-PAGE. x * 60700 + x * 40900, GDHA/CB. x * 60700 + x * 42200, GDHA/CLB, SDS-PAGE Klebsiella pneumoniae

Synonyms

EC Number Synonyms Comment Organism
4.2.1.30 B12-dependent glycerol dehydratase
-
Klebsiella pneumoniae
4.2.1.30 GDH
-
Klebsiella pneumoniae

General Information

EC Number General Information Comment Organism
4.2.1.30 malfunction GDH loses most of its beta-subunit after purification by either ion exchange chromatography or using a nickel chelate column Klebsiella pneumoniae