EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.9 | gene mmgA, encoded in the mmg operon, DNA and amino acid sequence determination and analysis, overexpression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) | Bacillus subtilis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.9 | 0.05 | - |
acetoacetyl-CoA | pH 6.0, 37°C | Bacillus subtilis | |
2.3.1.9 | 0.07 | - |
CoA | pH 6.0, 37°C | Bacillus subtilis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.3.1.9 | 42000 | - |
2 * 42000, recombinant His-tagged enzyme, SDS-PAGE | Bacillus subtilis |
2.3.1.9 | 84000 | - |
about, recombinant His-tagged enzyme, gel filtration | Bacillus subtilis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.9 | Bacillus subtilis | - |
gene mmgA | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.9 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Bacillus subtilis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.9 | acetoacetyl-CoA + CoA | the enzyme shows preference for the thiolytic reaction direction | Bacillus subtilis | 2 acetyl-CoA | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.3.1.9 | dimer | 2 * 42000, recombinant His-tagged enzyme, SDS-PAGE | Bacillus subtilis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.9 | acetoacetyl-CoA thiolase | - |
Bacillus subtilis |
2.3.1.9 | MmgA | - |
Bacillus subtilis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.9 | 37 | - |
assay at | Bacillus subtilis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.9 | 80 | - |
acetoacetyl-CoA | pH 6.0, 37°C | Bacillus subtilis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.9 | 6 | - |
assay at | Bacillus subtilis |