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Literature summary extracted from

  • Kurt, A.G.; Aytan, E.; Ozer, U.; Ates, B.; Geckil, H.
    Production of L-DOPA and dopamine in recombinant bacteria bearing the Vitreoscilla hemoglobin gene (2009), Biotechnol. J., 4, 1077-1088.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.99.2 additional information Vitreoscilla hemoglobin positive strains (vgb+ strains) have substantially higher TPL activity than the respective wild-type hosts or the strains bearing a comparable plasmid without vgb (the vgb- strains) Pantoea agglomerans
4.1.99.2 additional information Vitreoscilla hemoglobin positive strains (vgb+ strains) have substantially higher TPL activity than the respective wild-type hosts or the strains bearing a comparable plasmid without vgb (the vgb- strains) Citrobacter freundii

Organism

EC Number Organism UniProt Comment Textmining
4.1.99.2 Citrobacter freundii
-
 NRRL B-2643
-
4.1.99.2 Citrobacter freundii NRRL B-2643
-
 NRRL B-2643
-
4.1.99.2 Pantoea agglomerans
-
 NRRL B-3466
-
4.1.99.2 Pantoea agglomerans NRRL B-3466
-
 NRRL B-3466
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.99.2 0.017
-
 at the 24 h culture phase, wild-type strain Pantoea agglomerans
4.1.99.2 0.031
-
 at the 24 h culture phase, wild-type strain Citrobacter freundii
4.1.99.2 0.032
-
 at the 12 h culture phase, wild-type strain Citrobacter freundii
4.1.99.2 0.058
-
 at the 12 h culture phase, vgb- strain Citrobacter freundii
4.1.99.2 0.067
-
 at the 24 h culture phase, vgb- strain Citrobacter freundii
4.1.99.2 0.08
-
 at the 12 h culture phase, vgb+ strain Citrobacter freundii
4.1.99.2 0.098
-
 at the 24 h culture phase, vgb- strain Pantoea agglomerans
4.1.99.2 0.371
-
 at the 24 h culture phase, vgb+ strain Citrobacter freundii
4.1.99.2 0.513
-
 at the 24 h culture phase, vgb+ strain Pantoea agglomerans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.99.2 catechol + pyruvate + NH3 TPL is well-correlated to cytoplasmic 3,4-dihydroxyphenyl-L-alanine levels Citrobacter freundii 3,4-dihydroxyphenyl-L-alanine
-
 r
4.1.99.2 catechol + pyruvate + NH3 TPL is well-correlated to cytoplasmic 3,4-dihydroxyphenyl-L-alanine levels Citrobacter freundii NRRL B-2643 3,4-dihydroxyphenyl-L-alanine
-
 r
4.1.99.2 L-tyrosine + H2O TPL is well-correlated to cytoplasmic 3,4-dihydroxyphenyl-L-alanine levels Pantoea agglomerans 3,4-dihydroxyphenyl-L-alanine
-
 ?
4.1.99.2 L-tyrosine + H2O TPL is well-correlated to cytoplasmic 3,4-dihydroxyphenyl-L-alanine levels Pantoea agglomerans NRRL B-3466 3,4-dihydroxyphenyl-L-alanine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.99.2 TPL
-
 Pantoea agglomerans
4.1.99.2 TPL
-
 Citrobacter freundii
4.1.99.2 tyrosine phenol-lyase
-
 Pantoea agglomerans
4.1.99.2 tyrosine phenol-lyase
-
 Citrobacter freundii

Expression

EC Number Organism Comment Expression
4.1.99.2 Pantoea agglomerans L-tyrosine addition only has a positive effect in terms of TPL induction for cell cultures harvested after 12 h up
4.1.99.2 Citrobacter freundii L-tyrosine addition only has a positive effect in terms of TPL induction for cell cultures harvested after 12 h up