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Literature summary extracted from

  • Ramanan, R.; Kannan, K.; Vinayagamoorthy, N.; Ramkumar, K.; Sivanesan, S.; Chakrabarti, T.
    Purification and characterization of a novel plant-type carbonic anhydrase from Bacillus subtilis (2009), Biotechnol. Bioprocess Eng., 14, 32-37.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.1 acetazolamide 62% residual activity at 1 mM Bacillus subtilis
4.2.1.1 Ca2+ 72% residual activity at 1 mM Bacillus subtilis
4.2.1.1 Cl- 10% residual activity at 1 mM Bacillus subtilis
4.2.1.1 EDTA complete inhibition at 1 mM Bacillus subtilis
4.2.1.1 HCO3- 7% residual activity at 1 mM Bacillus subtilis
4.2.1.1 Hg2+ complete inhibition at 1 mM Bacillus subtilis
4.2.1.1 Mn2+ 73% residual activity at 1 mM Bacillus subtilis
4.2.1.1 NO2- 41% residual activity at 1 mM Bacillus subtilis
4.2.1.1 NO3- 36% residual activity at 1 mM Bacillus subtilis
4.2.1.1 Pb2+ 33% residual activity at 1 mM Bacillus subtilis
4.2.1.1 sulfanilamide
-
 Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.1 9.09
-
 4-nitrophenyl acetate in 0.1 M Tris-HCl buffer, pH 8.3, and 37°C Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.1 Co2+ 305% activity at 1 mM Bacillus subtilis
4.2.1.1 Cu2+ 165% activity at 1 mM Bacillus subtilis
4.2.1.1 Fe3+ 281% activity at 1 mM Bacillus subtilis
4.2.1.1 Mg2+ 107% activity at 1 mM Bacillus subtilis
4.2.1.1 SO42- 126% activity at 1 mM Bacillus subtilis
4.2.1.1 Zn2+ 473% activity at 1 mM Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.1 37000
-
 SDS-PAGE Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.1 Bacillus subtilis
-
-
-
4.2.1.1 Bacillus subtilis SA3
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.1 Sephadex G-75 gel filtration, DEAE cellulose column chromatography, and Sepharose-4B-L tyrosine sulfanilamide column chromatography Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.1 1.62
-
 crude extract, using 4-nitrophenyl acetate as substrate, at pH 8.3 and 37°C Bacillus subtilis
4.2.1.1 737.8
-
 after 456.6fold purification, using 4-nitrophenyl acetate as substrate, at pH 8.3 and 37°C Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.1 4-nitrophenyl acetate + H2O
-
 Bacillus subtilis 4-nitrophenol + acetate
-
 ?
4.2.1.1 4-nitrophenyl acetate + H2O
-
 Bacillus subtilis SA3 4-nitrophenol + acetate
-
 ?
4.2.1.1 CO2 + H2O
-
 Bacillus subtilis H2CO3
-
 r
4.2.1.1 CO2 + H2O
-
 Bacillus subtilis SA3 H2CO3
-
 r

Synonyms

EC Number Synonyms Comment Organism
4.2.1.1 carbonate hydro-lyase
-
 Bacillus subtilis
4.2.1.1 carbonic anhydrase
-
 Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.1 37
-
-
 Bacillus subtilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.1 60
-
 the enzyme is not stable at temperatures of 60°C and above Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.1 8.3
-
-
 Bacillus subtilis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.1.1 4 13 activity is completely lost at a moderately acidic pH of 4.0 and a highly basic pH of 13.0 Bacillus subtilis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.1.1 0.099
-
 sulfanilamide in 0.1 M Tris-HCl buffer, pH 8.3, and 37°C Bacillus subtilis
4.2.1.1 0.22
-
 acetazolamide in 0.1 M Tris-HCl buffer, pH 8.3, and 37°C Bacillus subtilis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.1.1 Bacillus subtilis isoelectric focusing
-
 6.1