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Literature summary extracted from

  • Ryan, J.; Clark, D.
    P450cam biocatalysis in surfactant-stabilized two-phase emulsions (2008), Biotechnol. Bioeng., 99, 1311-1319.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.15.1 bis(2-ethylhexyl) sulfosuccinate improves the initial activity of P450cam in two-phase emulsions with initial camphor concentrations of 5-15 mM. P450cam is activated in the surfactant-free emulsions, and addition of bis(2-ethylhexyl) sulfosuccinate sodium salt improves the activity even further, at least over the range of camphor concentrations for which initial rates are readily measurable in all media. The largest rate enhancement is 4.5fold. Nearly 50times more product is formed in the surfactant-stabilized emulsions than is achieved in aqueous buffer Pseudomonas putida

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.15.1 plasmids transformed into Escherichia coli Bl21(DE3) competent cells Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.15.1 additional information emulsions formed by strongly agitating the mixture with a vortexer denatured protein, P450cam has a complete loss of activity when incubated under these conditions for more than 5 min prior to the initiation of the reaction Pseudomonas putida

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.14.15.1 hexane P450cam is stable and retains 80% of its initial activity after 1 h in a hexane/water emulsion at agitation speeds of less than 250 rpm Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.1 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.15.1 by anion exchange, hydrophobic interaction and gel filtration Pseudomonas putida

Storage Stability

EC Number Storage Stability Organism
1.14.15.1 -80°C Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.1 (+)-camphor + O2 + reduced putidaredoxin
-
 Pseudomonas putida exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
-
 ?
1.14.15.1 (R)-exo-5-hydroxycamphor + O2 + reduced putidaredoxin
-
 Pseudomonas putida 2,5-diketocamphane + oxidized putidaredoxin + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.15.1 P450cam
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.1 cytochrome m optimal ratio of putidaredoxin reductase, putidaredoxin, cytochrome m is 1:10:2 Pseudomonas putida
1.14.15.1 putidaredoxin optimal ratio of putidaredoxin reductase, putidaredoxin, cytochrome m is 1:10:2 Pseudomonas putida