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Literature summary extracted from

  • Berheide, M.; Peper, S.; Kara, S.; Long, W.S.; Schenkel, S.; Pohl, M.; Niemeyer, B.; Liese, A.
    Influence of the hydrostatic pressure and pH on the asymmetric 2-hydroxyketone formation catalyzed by Pseudomonas putida benzoylformate decarboxylase and variants thereof (2010), Biotechnol. Bioeng., 106, 18-26.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.7 expressed in Escherichia coli strain SG13009 Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.7 A460I for this variant the highest amounts of (S)-2-hydroxypropiophenone-product can be detected at low benzaldehyde concentrations and slightly acidic conditions at pH 6.5 Pseudomonas putida
4.1.1.7 A460I/F464I for this variant the highest amounts of (S)-2-hydroxypropiophenone-product can be detected at low benzaldehyde concentrations and slightly acidic conditions at pH 6.5 Pseudomonas putida
4.1.1.7 F464I for this variant the highest amounts of (S)-2-hydroxypropiophenone-product can be detected at low benzaldehyde concentrations and slightly acidic conditions at pH 6.5 Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.7 Mg2+ required Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.7 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.7 Q-Sepharose column chromatography, Superdex 200 gel filtration Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.7 benzaldehyde + acetaldehyde
-
 Pseudomonas putida (S)-2-hydroxypropiophenone
-
 ?
4.1.1.7 benzylformate
-
 Pseudomonas putida benzaldehyde + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.7 BFD
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.7 thiamine diphosphate dependent on Pseudomonas putida