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Literature summary extracted from

  • Tham, S.J.; Chang, C.D.; Huang, H.J.; Lee, Y.F.; Huang, T.S.; Chang, C.C.
    Biochemical characterization of an acid phosphatase from Thermus thermophilus (2010), Biosci. Biotechnol. Biochem., 74, 727-735.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.2 His-tagged version expressed in Escherichia coli BL21(DE3) Thermus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.2 ascorbate 1 mM: 60% inhibition Thermus thermophilus
3.1.3.2 CuCl2
-
Thermus thermophilus
3.1.3.2 EDTA
-
Thermus thermophilus
3.1.3.2 EGTA
-
Thermus thermophilus
3.1.3.2 additional information not inhibited by 10 mM sodium tartrate, 1 mM NaF, 1 mM 2-mercaptoethanol Thermus thermophilus
3.1.3.2 ZnCl2
-
Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.2 1.4
-
p-nitrophenyl phosphate
-
Thermus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.2 Ca2+
-
Thermus thermophilus
3.1.3.2 Mg2+ Ca2+ has a more important role than Mg2+ in enzymatic activities Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.2 28400
-
probably tetramer 4 * 28400, SDS-PAGE, calculated Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Thermus thermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2 immobilized metal ion affinity chromatography (Ni2+), gel filtration Thermus thermophilus

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.1.3.2 zymogram analysis after SDS-PAGE Thermus thermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.2 670000
-
substrate p-nitrophenyl phosphate Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 p-nitrophenyl phosphate + H2O substrate preference for p-nitrophenyl phosphate, further substrates: 5-bromo-4-chloro-3-indolyl phosphate, ATP, dATP, dTTP, dGTP, dCTP, less preference for phytate, glycerol-2-phosphate Thermus thermophilus p-nitrophenol + phosphate
-
?

Subunits

EC Number Subunits Comment Organism
3.1.3.2 oligomer probably tetramer 4 * 28400, SDS-PAGE, calculated Thermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
3.1.3.2 acid phosphatase
-
Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.2 70
-
-
Thermus thermophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.3.2 40 90
-
Thermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.2 additional information
-
after 15 min at 70°C: 44% residual activity, after 15 min at 90°C: 22% residual activity Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 6
-
acetate buffer Thermus thermophilus
3.1.3.2 6.5
-
Hepes buffer, pH 6.1-pH 7.2 with Tris buffer Thermus thermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.2 3.6 9.1
-
Thermus thermophilus