EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.3.2 | His-tagged version expressed in Escherichia coli BL21(DE3) | Thermus thermophilus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.2 | ascorbate | 1 mM: 60% inhibition | Thermus thermophilus | |
3.1.3.2 | CuCl2 | - |
Thermus thermophilus | |
3.1.3.2 | EDTA | - |
Thermus thermophilus | |
3.1.3.2 | EGTA | - |
Thermus thermophilus | |
3.1.3.2 | additional information | not inhibited by 10 mM sodium tartrate, 1 mM NaF, 1 mM 2-mercaptoethanol | Thermus thermophilus | |
3.1.3.2 | ZnCl2 | - |
Thermus thermophilus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.2 | 1.4 | - |
p-nitrophenyl phosphate | - |
Thermus thermophilus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.2 | Ca2+ | - |
Thermus thermophilus | |
3.1.3.2 | Mg2+ | Ca2+ has a more important role than Mg2+ in enzymatic activities | Thermus thermophilus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.3.2 | 28400 | - |
probably tetramer 4 * 28400, SDS-PAGE, calculated | Thermus thermophilus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.2 | Thermus thermophilus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.3.2 | immobilized metal ion affinity chromatography (Ni2+), gel filtration | Thermus thermophilus |
EC Number | Renatured (Comment) | Organism |
---|---|---|
3.1.3.2 | zymogram analysis after SDS-PAGE | Thermus thermophilus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.3.2 | 670000 | - |
substrate p-nitrophenyl phosphate | Thermus thermophilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.2 | p-nitrophenyl phosphate + H2O | substrate preference for p-nitrophenyl phosphate, further substrates: 5-bromo-4-chloro-3-indolyl phosphate, ATP, dATP, dTTP, dGTP, dCTP, less preference for phytate, glycerol-2-phosphate | Thermus thermophilus | p-nitrophenol + phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.3.2 | oligomer | probably tetramer 4 * 28400, SDS-PAGE, calculated | Thermus thermophilus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.3.2 | acid phosphatase | - |
Thermus thermophilus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.2 | 70 | - |
- |
Thermus thermophilus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.2 | 40 | 90 | - |
Thermus thermophilus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.2 | additional information | - |
after 15 min at 70°C: 44% residual activity, after 15 min at 90°C: 22% residual activity | Thermus thermophilus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.2 | 6 | - |
acetate buffer | Thermus thermophilus |
3.1.3.2 | 6.5 | - |
Hepes buffer, pH 6.1-pH 7.2 with Tris buffer | Thermus thermophilus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.2 | 3.6 | 9.1 | - |
Thermus thermophilus |