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Literature summary extracted from

  • Konishi, T.; Miyazaki, Y.; Yamakawa, S.; Iwai, H.; Satoh, S.; Ishii, T.
    Purification and biochemical characterization of recombinant rice UDP-arabinopyranose mutase generated in insect cells (2010), Biosci. Biotechnol. Biochem., 74, 191-194.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.4.99.30 additional information the combination of rUAM1, rUAM2, and rUAM3 increases activity 1.7fold as compared with that of the sum total of rUAM1, rUAM2, and rUAM3. This suggests that UAM isoenzymes work synergistically to increase activity Oryza sativa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.99.30 expression in insect cells Oryza sativa

General Stability

EC Number General Stability Organism
5.4.99.30 rUMA1 expressed in insect cells retains activity after repeated cycles of freezing and thawing Oryza sativa

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.4.99.30 UDP competitive inhibition, lower affinity for enzyme than substrate Oryza sativa
5.4.99.30 UDP-Gal activity decreases by 40% under pretreatment for 3 h Oryza sativa
5.4.99.30 UDP-Glc activity decreases by 40% under pretreatment for 3 h Oryza sativa
5.4.99.30 UDP-Xyl activity decreases by 40% under pretreatment for 3 h Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.99.30 0.0228
-
UDP-beta-L-arabinofuranose pH 6.8, 25°C Oryza sativa
5.4.99.30 0.0454
-
UDP-beta-L-arabinopyranose pH 6.8, 25°C Oryza sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.4.99.30 additional information
-
native mutase is likely to exist as a complex composed of several different UDP-arabinopyranose mutases Oryza sativa
5.4.99.30 40000
-
x * 40000, SDS-PAGE Oryza sativa
5.4.99.30 41349
-
x * 41349, calculated from sequence Oryza sativa

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.30 Oryza sativa Q6Z4G3
-
-
5.4.99.30 Oryza sativa Q8H8T0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.99.30
-
Oryza sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.4.99.30 seedling
-
Oryza sativa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.30 UDP-beta-L-arabinofuranose the ratio of UDP-beta-L-arabinopyranose to UDP-beta-L-arabinofuranose reaches a plateau at 93:7 from both pyranose- and furanose-forming directions Oryza sativa UDP-beta-L-arabinopyranose
-
r

Subunits

EC Number Subunits Comment Organism
5.4.99.30 ? x * 40000, SDS-PAGE Oryza sativa
5.4.99.30 ? x * 41349, calculated from sequence Oryza sativa

Synonyms

EC Number Synonyms Comment Organism
5.4.99.30 UAM1
-
Oryza sativa
5.4.99.30 UAM3
-
Oryza sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.4.99.30 50
-
-
Oryza sativa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5.4.99.30 30 60 30°C: about 50% of maximal activity, 60°C: about 85% of maximal activity Oryza sativa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.4.99.30 25
-
80 h, 50% loss of activity Oryza sativa
5.4.99.30 50
-
1 h, 50% loss of activity Oryza sativa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.4.99.30 0.185
-
UDP-beta-L-arabinopyranose pH 6.8, 25°C Oryza sativa
5.4.99.30 0.474
-
UDP-beta-L-arabinofuranose pH 6.8, 25°C Oryza sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4.99.30 6
-
pyranose forming activity Oryza sativa
5.4.99.30 6.5
-
furanose-forming activity Oryza sativa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.4.99.30 4 8 pH 4.0: about 40% of maximal activity, pH 8.0: about 55% of maximal activity, pyranose forming activity Oryza sativa
5.4.99.30 5 8 pH 5.0: about 50% of maximal activity, pH 8.0: about 50% of maximal activity, furanose-forming activity Oryza sativa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5.4.99.30 1.4
-
UDP pH 6.8, 25°C Oryza sativa

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.4.99.30 20.8
-
UDP-beta-L-arabinofuranose pH 6.8, 25°C Oryza sativa
5.4.99.30 40.8
-
UDP-beta-L-arabinopyranose pH 6.8, 25°C Oryza sativa