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Literature summary extracted from

  • Kino, K.; Kotanaka, Y.; Arai, T.; Yagasaki, M.
    A novel L-amino acid ligase from Bacillus subtilis NBRC3134, a microorganism producing peptide-antibiotic rhizocticin (2009), Biosci. Biotechnol. Biochem., 73, 901-907.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.48 expression in Escherichia coli Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.48 Mg2+ required. When Mg2+ is replaced by Mn2+ and Co2+, L-amino acid ligase activity is decreased Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.48 45000
-
 2 * 45000, SDS-PAGE Bacillus subtilis
6.3.2.48 46344
-
 2 * 46344, calculated from sequence Bacillus subtilis
6.3.2.48 80000
-
 gel filtration Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.48 additional information Bacillus subtilis the enzyme is expected to be involved in the biosynthesis of rhizocticin (dipeptide or tripeptide antibiotics that commonly possess L-arginyl-L-2-amino-5-phosphono-3-cis-pentenoic acid) ?
-
 ?
6.3.2.48 additional information Bacillus subtilis NBRC3134 the enzyme is expected to be involved in the biosynthesis of rhizocticin (dipeptide or tripeptide antibiotics that commonly possess L-arginyl-L-2-amino-5-phosphono-3-cis-pentenoic acid) ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.48 Bacillus subtilis B5UAT8
-
-
6.3.2.48 Bacillus subtilis NBRC3134 B5UAT8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.48
-
 Bacillus subtilis

Reaction

EC Number Reaction Comment Organism Reaction ID
6.3.2.48 ATP + L-arginine + an L-amino acid = ADP + phosphate + an L-arginyl-L-amino acid Xaa is an arbitrary amino acid except Pro (Arg, Lys, His, Gln, Asn, Glu, Asp, Ala, Ser, Thr, Gly, Val, Leu, Ile, Met, Cys, Phe, Trp, Tyr) Bacillus subtilis
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.48 ATP + 2 L-Arg the enzyme synthesizes hetero-dipeptides. Arg is present at the N-terminus. The enzyme synthesizes Arg-Arg, when only Arg is used as substrate, but preferentially synthesizes Arg-Xaa when Arg and other amino acids are used as substrates. RizA has strict substrate specificity toward L-arginine as the N-terminal substrate. The substrate specificity at the C-terminus is very relaxed. Hetero-dipeptide consisting of Arg and Pro is not detected. The enzyme does not use D-form amino acids as substrates. No tripeptides and no longer peptides are detected. The substrate specificity at the C-terminus is relaxed Bacillus subtilis ADP + phosphate + L-Arg-L-Arg
-
 ?
6.3.2.48 ATP + 2 L-Arg the enzyme synthesizes hetero-dipeptides. Arg is present at the N-terminus. The enzyme synthesizes Arg-Arg, when only Arg is used as substrate, but preferentially synthesizes Arg-Xaa when Arg and other amino acids are used as substrates. RizA has strict substrate specificity toward L-arginine as the N-terminal substrate. The substrate specificity at the C-terminus is very relaxed. Hetero-dipeptide consisting of Arg and Pro is not detected. The enzyme does not use D-form amino acids as substrates. No tripeptides and no longer peptides are detected. The substrate specificity at the C-terminus is relaxed Bacillus subtilis NBRC3134 ADP + phosphate + L-Arg-L-Arg
-
 ?
6.3.2.48 ATP + L-Arg + L-2-amino-5-phosphono-3-cis-pentenoate
-
 Bacillus subtilis ADP + phosphate + L-Arg-L-2-amino-5-phosphono-3-cis-pentenoate
-
 ?
6.3.2.48 ATP + L-Arg + L-2-amino-5-phosphono-3-cis-pentenoate
-
 Bacillus subtilis NBRC3134 ADP + phosphate + L-Arg-L-2-amino-5-phosphono-3-cis-pentenoate
-
 ?
6.3.2.48 ATP + L-Arg + L-Ala
-
 Bacillus subtilis ADP + phosphate + L-Arg-L-Ala
-
 ?
6.3.2.48 ATP + L-Arg + L-Ala
-
 Bacillus subtilis NBRC3134 ADP + phosphate + L-Arg-L-Ala
-
 ?
6.3.2.48 ATP + L-Arg + L-His
-
 Bacillus subtilis ADP + phosphate + L-Arg-L-His
-
 ?
6.3.2.48 ATP + L-Arg + L-His
-
 Bacillus subtilis NBRC3134 ADP + phosphate + L-Arg-L-His
-
 ?
6.3.2.48 ATP + L-Arg + L-Ser
-
 Bacillus subtilis ADP + phosphate + L-Arg-L-Ser
-
 ?
6.3.2.48 ATP + L-Arg + L-Xaa the enzyme synthesizes hetero-dipeptides. Arg is present at the N-terminus. The enzyme synthesizes Arg-Arg, when only Arg is used as substrate, but preferentially synthesizes Arg-Xaa when Arg and other amino acids are used as substrates. RizA has strict substrate specificity toward L-arginine as the N-terminal substrate. Hetero-dipeptide consisting of Arg and Pro is not detected. The enzyme does not use D-form amino acids as substrates. No tripeptides and no longer peptides are detected. The substrate specificity at the C-terminus is relaxed Bacillus subtilis ADP + phosphate + L-Arg-L-Xaa
-
 ?
6.3.2.48 additional information the enzyme is expected to be involved in the biosynthesis of rhizocticin (dipeptide or tripeptide antibiotics that commonly possess L-arginyl-L-2-amino-5-phosphono-3-cis-pentenoic acid) Bacillus subtilis ?
-
 ?
6.3.2.48 additional information the enzyme is expected to be involved in the biosynthesis of rhizocticin (dipeptide or tripeptide antibiotics that commonly possess L-arginyl-L-2-amino-5-phosphono-3-cis-pentenoic acid) Bacillus subtilis NBRC3134 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.2.48 homodimer 2 * 45000, SDS-PAGE Bacillus subtilis
6.3.2.48 homodimer 2 * 46344, calculated from sequence Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
6.3.2.48 RizA
-
 Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.2.48 30
-
 assay at Bacillus subtilis
6.3.2.48 37
-
-
 Bacillus subtilis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
6.3.2.48 30 50 30°C: about 50% of maximal activity, 50°C: about 55% of maximal activity. Significant decrease of activity above 50°C Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.48 8
-
 assay at Bacillus subtilis
6.3.2.48 9.5
-
-
 Bacillus subtilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.3.2.48 9 10 pH 9.0: about 60% of maximal activity, pH 10.0: about 50% of maximal activity. Sharp decrease of activity below pH 9.0 and above pH 10.0 Bacillus subtilis