Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Fujieda, N.; Mori, M.; Ikeda, T.; Kano, K.
    The silent form of quinohemoprotein amine dehydrogenase from Paracoccus denitrificans (2009), Biosci. Biotechnol. Biochem., 73, 524-529.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.4.9.1 benzylamine activates sQH-AmDH Paracoccus denitrificans
1.4.9.1 Dithionite rapidly activates sQH-AmDH, activation process involves a reduction process Paracoccus denitrificans
1.4.9.1 dithiothreitol rapidly activates sQH-AmDH, activation process involves a reduction process Paracoccus denitrificans
1.4.9.1 n-butylamine activates sQH-AmDH Paracoccus denitrificans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.9.1 36500
-
 SDS-PAGE, heme 2 shows two bands Paracoccus denitrificans
1.4.9.1 59500
-
 SDS-PAGE, heme 2 shows two bands Paracoccus denitrificans

Organism

EC Number Organism UniProt Comment Textmining
1.4.9.1 Paracoccus denitrificans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.9.1 ion-exchange chromatography with DEAE-Toyopearl resin Paracoccus denitrificans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.9.1 additional information
-
 heme 2 does not show any enzymatic activity for amine oxidation. Heme 2 is slowly activated in 10-30 h during incubation with n-butylamine. Enzyme is faster activated with benzylamine Paracoccus denitrificans
1.4.9.1 additional information
-
 sQH-AmDH is not electrochemically activated by direct electrolytic reduction with a column electrode at -0.5 V, but it is electrochemically activated by mediated electrolytic reduction with a column electrode Paracoccus denitrificans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.9.1 benzylamine + H2O + ferricyanide
-
 Paracoccus denitrificans benzaldehyde + NH3 + reduced ferricyanide
-
 ?
1.4.9.1 n-butylamine + H2O + ferricyanide
-
 Paracoccus denitrificans butanal + NH3 + reduced ferricyanide
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.9.1 heterotrimer gel filtration, mass spectroscopy and UV-VIS spectroscopy: the gamma subunit of sQH-AmDH shows a sharp peak at 390 nm in UV-VIS spectrum clearly distinguishable from that of QH-AmDH. Electrospray ionization mass spectrometric analysis shows that the molecular mass of the gamma subunit of sQH-AmDH is larger than that of QH-AmDH by 15.6. The data suggest that the cysteine tryptophylquinone-like moiety (catalytic center) of the gamma subunit of sQH-AmDH is an oxime Paracoccus denitrificans

Synonyms

EC Number Synonyms Comment Organism
1.4.9.1 Heme 2 inactive form of QH-AmDH Paracoccus denitrificans
1.4.9.1 quinohemoprotein amine dehydrogenase
-
 Paracoccus denitrificans
1.4.9.1 sQH-AmDH silent form of QH-AmDH Paracoccus denitrificans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.9.1 30
-
 assay at Paracoccus denitrificans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.9.1 7.5
-
 assay at Paracoccus denitrificans