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Literature summary extracted from

  • Senoura, T.; Taguchi, H.; Ito, S.; Hamada, S.; Matsui, H.; Fukiya, S.; Yokota, A.; Watanabe, J.; Wasaki, J.; Ito, S.
    Identification of the cellobiose 2-epimerase gene in the genome of Bacteroides fragilis NCTC 9343 (2009), Biosci. Biotechnol. Biochem., 73, 400-406.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.3.11 expression in Escherichia coli Bacteroides fragilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.3.11 3.7 5 cellobiose recombinant enzyme, 100 mM sodium phosphate, 20 mM cellobiose, pH 7.5, 30°C Bacteroides fragilis
5.1.3.11 6.56
-
 lactose recombinant enzyme, 100 mM sodium phosphate, 20 mM lactose, pH 7.5, 30°C Bacteroides fragilis
5.1.3.11 13.8
-
 cellobiose recombinant enzyme, 100 mM sodium phosphate, 20 mM cellobiose, pH 7.5, 30°C Ruminococcus albus
5.1.3.11 33
-
 lactose recombinant enzyme, 100 mM sodium phosphate, 20 mM lactose, pH 7.5, 30°C Ruminococcus albus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.3.11 41100
-
 determined by SDS-PAGE, the mass discrepancy putatively results from partial hydrolysis at the C-terminus during expression or in the purification steps Bacteroides fragilis
5.1.3.11 45780
-
 calculated by amino acid sequence, confirmed by SDS-PAGE Bacteroides fragilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.1.3.11 beta-mannobiose Bacteroides fragilis
-
 4-O-beta-D-mannopyranosyl-D-glucose putative product r
5.1.3.11 cellobiose Ruminococcus albus
-
 4-O-beta-D-glucopyranosyl-D-mannose
-
 r
5.1.3.11 cellobiose Bacteroides fragilis
-
 4-O-beta-D-glucopyranosyl-D-mannose
-
 r
5.1.3.11 globotriose Bacteroides fragilis
-
 O-alpha-D-galactopyranosyl-(1,4)-O-beta-D-galactopyranosyl-(1,4)-D-mannose putative product r
5.1.3.11 lactose Ruminococcus albus
-
 4-O-beta-D-galactopyranosyl-D-mannose
-
 r
5.1.3.11 lactose Bacteroides fragilis
-
 4-O-beta-D-galactopyranosyl-D-mannose
-
 r
5.1.3.11 additional information Bacteroides fragilis recombinant enzyme catalyzes a hydroxyl stereoisomerism at the C2-positions of the reducing terminal glucose and at the mannose moiety of cello-oligosaccharides: lactose, beta-mannobiose and globotriose ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.11 Bacteroides fragilis Q5LH66
-
-
5.1.3.11 Ruminococcus albus P0DKY4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.3.11 by anion exchange chromatography, enzyme is further purified on a hydroxyapatite column Bacteroides fragilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.1.3.11 28.4
-
 recombinant enzyme, substrate is lactose Ruminococcus albus
5.1.3.11 29.9
-
 crude extract after expression in Escherichia coli cells Bacteroides fragilis
5.1.3.11 51
-
 recombinant enzyme, substrate is cellobiose Ruminococcus albus
5.1.3.11 58.3
-
 recombinant enzyme after first purification step on a DEAE Sepharose FF column Bacteroides fragilis
5.1.3.11 61.8
-
 recombinant enzyme after second purification step on a hydroxyapatite column, substrate is cellobiose Bacteroides fragilis
5.1.3.11 79.7
-
 recombinant enzyme after second purification step on a hydroxyapatite column, substrate is lactose Bacteroides fragilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.11 beta-mannobiose
-
 Bacteroides fragilis 4-O-beta-D-mannopyranosyl-D-glucose putative product r
5.1.3.11 cellobiose
-
 Ruminococcus albus 4-O-beta-D-glucopyranosyl-D-mannose
-
 r
5.1.3.11 cellobiose
-
 Bacteroides fragilis 4-O-beta-D-glucopyranosyl-D-mannose
-
 r
5.1.3.11 globotriose
-
 Bacteroides fragilis O-alpha-D-galactopyranosyl-(1,4)-O-beta-D-galactopyranosyl-(1,4)-D-mannose putative product r
5.1.3.11 lactose
-
 Ruminococcus albus 4-O-beta-D-galactopyranosyl-D-mannose
-
 r
5.1.3.11 lactose
-
 Bacteroides fragilis 4-O-beta-D-galactopyranosyl-D-mannose
-
 r
5.1.3.11 additional information recombinant enzyme catalyzes a hydroxyl stereoisomerism at the C2-positions of the reducing terminal glucose and at the mannose moiety of cello-oligosaccharides: lactose, beta-mannobiose and globotriose Bacteroides fragilis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.1.3.11 cellobiose 2-epimerase
-
 Ruminococcus albus
5.1.3.11 cellobiose 2-epimerase
-
 Bacteroides fragilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.1.3.11 30
-
 assay at Bacteroides fragilis
5.1.3.11 45
-
 optimum Bacteroides fragilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.1.3.11 52.1
-
 lactose recombinant enzyme, 100 mM sodium phosphate, 20 mM lactose, pH 7.5, 30°C Ruminococcus albus
5.1.3.11 63.8
-
 cellobiose recombinant enzyme, 100 mM sodium phosphate, 20 mM cellobiose, pH 7.5, 30°C Ruminococcus albus
5.1.3.11 67.6
-
 cellobiose recombinant enzyme, 100 mM sodium phosphate, 20 mM cellobiose, pH 7.5, 30°C Bacteroides fragilis
5.1.3.11 79.5
-
 lactose recombinant enzyme, 100 mM sodium phosphate, 20 mM lactose, pH 7.5, 30°C Bacteroides fragilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.3.11 7.5
-
 assay at Bacteroides fragilis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.1.3.11 1.58
-
 lactose recombinant enzyme, 100 mM sodium phosphate, 20 mM lactose, pH 7.5, 30°C Ruminococcus albus
5.1.3.11 4.62
-
 cellobiose recombinant enzyme, 100 mM sodium phosphate, 20 mM cellobiose, pH 7.5, 30°C Ruminococcus albus
5.1.3.11 12.1
-
 lactose recombinant enzyme, 100 mM sodium phosphate, 20 mM lactose, pH 7.5, 30°C Bacteroides fragilis
5.1.3.11 18.1
-
 cellobiose recombinant enzyme, 100 mM sodium phosphate, 20 mM cellobiose, pH 7.5, 30°C Bacteroides fragilis