Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yamamoto, S.; Morihara, Y.; Wakayama, M.; Tachiki, T.
    Repeated batch production of theanine by coupled fermentation with energy transfer using membrane-enclosed gamma-glutamylmethylamide synthetase and dried yeast cells (2009), Biosci. Biotechnol. Biochem., 73, 2800-2802.
    View publication on PubMed

Application

EC Number Application Comment Organism
6.3.4.12 synthesis the enzyme is enclosed with dried Saccharomyces cerevisiae cells in a dialysis membrane tube to produce theanine from glutamic acid and ethylamine to 100% conversion rate. Six repeats of the reaction are possible in presence of NAD+, overview Methylovorus mays

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.4.12 expression in Escherichia coli strain AD494(DE3) Methylovorus mays

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.4.12 Mg2+
-
 Methylovorus mays

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.12 Methylovorus mays
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.4.12 additional information freeze-dried cells Methylovorus mays
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.12 L-glutamic acid + ethylamine
-
 Methylovorus mays theanine i.e. gamma-glutamylethylamide ?

Synonyms

EC Number Synonyms Comment Organism
6.3.4.12 gamma-Glutamylmethylamide synthetase
-
 Methylovorus mays
6.3.4.12 GMAS
-
 Methylovorus mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.4.12 7
-
 assay at Methylovorus mays