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Literature summary extracted from
- The biological function of the bacterial isochorismatase-like hydrolase SttH (2009), Biosci. Biotechnol. Biochem., 73, 2494-2500.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.5.2.19 | expression in Escherichia coli | Streptomyces albulus |
| 3.5.2.19 | expression in Escherichia coli | Streptomyces noursei |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.5.2.19 | C158S | no activity detected | Streptomyces noursei |
| 3.5.2.19 | C176S | no activity detected | Streptomyces albulus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.2.19 | 1.3 | - |
streptothricin-F | pH 6.5, 30°C | Streptomyces noursei |  |
| 3.5.2.19 | 3.1 | - |
streptothricin-F | pH 6.5, 30°C | Streptomyces albulus | |
| 3.5.2.19 | 3.2 | - |
streptothricin-D | pH 6.5, 30°C | Streptomyces noursei | |
| 3.5.2.19 | 17.2 | - |
streptothricin-D | pH 6.5, 30°C | Streptomyces albulus | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.3.2.1 | isochorismate + H2O | Verticillium dahliae | isochorismatase converts isochorismate, in the presence of water, to 2,3-dihydroxybenzoate and pyruvate and inhibits salicylic acid formation | 2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate | - |
? | |
| 3.5.2.19 | streptothricin-D + H2O | Streptomyces noursei | - |
streptothricin-D acid | - |
? | |
| 3.5.2.19 | streptothricin-D + H2O | Streptomyces noursei NBRC15452 | - |
streptothricin-D acid | - |
? | |
| 3.5.2.19 | streptothricin-F + H2O | Streptomyces noursei | - |
streptothricin-F acid | - |
? | |
| 3.5.2.19 | streptothricin-F + H2O | Streptomyces noursei NBRC15452 | - |
streptothricin-F acid | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.3.2.1 | Verticillium dahliae | - |
highly, Vd1396-9, and weakly, Vs06-14, aggressive isolates from soil | - |
| 3.5.2.19 | Streptomyces albulus | - |
- |
- |
| 3.5.2.19 | Streptomyces albulus NBRC14147 | - |
- |
- |
| 3.5.2.19 | Streptomyces noursei | C5NU54 | - |
- |
| 3.5.2.19 | Streptomyces noursei NBRC15452 | C5NU54 | - |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.3.2.1 | additional information | proteomic analysis of highly, Vd1396-9, and weakly, Vs06-14, aggressive isolates from soil, isochorismatase family motifs occur in the secretome of five phytopathogenic but in no species of non-phytopathogenic filamentous ascomycete fungi | Verticillium dahliae | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.3.2.1 | isochorismate + H2O | - |
Verticillium dahliae | 2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate | - |
? | |
| 3.3.2.1 | isochorismate + H2O | isochorismatase converts isochorismate, in the presence of water, to 2,3-dihydroxybenzoate and pyruvate and inhibits salicylic acid formation | Verticillium dahliae | 2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate | - |
? | |
| 3.5.2.19 | streptothricin-D + H2O | - |
Streptomyces noursei | streptothricin-D acid | - |
? | |
| 3.5.2.19 | streptothricin-D + H2O | - |
Streptomyces albulus | streptothricin-D acid | the product is identified by reverse-phase HPLC | ? | |
| 3.5.2.19 | streptothricin-D + H2O | - |
Streptomyces noursei | streptothricin-D acid | the product is identified by reverse-phase HPLC | ? | |
| 3.5.2.19 | streptothricin-D + H2O | - |
Streptomyces albulus NBRC14147 | streptothricin-D acid | the product is identified by reverse-phase HPLC | ? | |
| 3.5.2.19 | streptothricin-D + H2O | - |
Streptomyces noursei NBRC15452 | streptothricin-D acid | - |
? | |
| 3.5.2.19 | streptothricin-D + H2O | - |
Streptomyces noursei NBRC15452 | streptothricin-D acid | the product is identified by reverse-phase HPLC | ? | |
| 3.5.2.19 | streptothricin-F + H2O | - |
Streptomyces noursei | streptothricin-F acid | - |
? | |
| 3.5.2.19 | streptothricin-F + H2O | - |
Streptomyces albulus | streptothricin-F acid | the product is identified by reverse-phase HPLC | ? | |
| 3.5.2.19 | streptothricin-F + H2O | - |
Streptomyces noursei | streptothricin-F acid | the product is identified by reverse-phase HPLC | ? | |
| 3.5.2.19 | streptothricin-F + H2O | - |
Streptomyces albulus NBRC14147 | streptothricin-F acid | the product is identified by reverse-phase HPLC | ? | |
| 3.5.2.19 | streptothricin-F + H2O | - |
Streptomyces noursei NBRC15452 | streptothricin-F acid | - |
? | |
| 3.5.2.19 | streptothricin-F + H2O | - |
Streptomyces noursei NBRC15452 | streptothricin-F acid | the product is identified by reverse-phase HPLC | ? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.2.19 | sttH | - |
Streptomyces albulus |
| 3.5.2.19 | sttH | - |
Streptomyces noursei |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.2.19 | 55 | - |
- |
Streptomyces albulus |
| 3.5.2.19 | 55 | - |
- |
Streptomyces noursei |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.2.19 | 7 | - |
- |
Streptomyces albulus |
| 3.5.2.19 | 7 | - |
- |
Streptomyces noursei |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.3.2.1 | metabolism | the biosynthesis of salicylic acid in plants occurs through phenylpropanoid and isochorismate pathways | Verticillium dahliae |
| 3.3.2.1 | physiological function | isochorismatase hydrolase may be acting as a plant-defense suppressor produced by the highly aggressive isolate to overcome host resistance | Verticillium dahliae |
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Release 2023.1 (January 2023)
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