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Literature summary extracted from

  • Nozaki, H.; Kuroda, S.; Watanabe, K.; Yokozeki, K.
    Cloning of the gene encoding alpha-methylserine hydroxymethyltransferase from Aminobacter sp. AJ110403 and Ensifer sp. AJ110404 and characterization of the recombinant enzyme (2008), Biosci. Biotechnol. Biochem., 72, 3002-3005.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.7 expression in Escherichia coli Aminobacter sp. AJ110403
2.1.2.7 expression in Escherichia coli Ensifer sp. AJ110404

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.7 0.09
-
 tetrahydrofolate
-
 Aminobacter sp. AJ110403
2.1.2.7 0.228
-
 tetrahydrofolate
-
 Ensifer sp. AJ110404
2.1.2.7 1.5
-
 2-methyl-L-serine
-
 Aminobacter sp. AJ110403
2.1.2.7 1.88
-
 2-methyl-L-serine
-
 Ensifer sp. AJ110404

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.7 73000
-
 gel filtration Aminobacter sp. AJ110403
2.1.2.7 80000
-
 gel filtration Ensifer sp. AJ110404

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.7 Aminobacter sp. AJ110403 B2DEU8
-
-
2.1.2.7 Ensifer sp. AJ110404 B2DEV1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.7 column chromatography, 16.4% yield Ensifer sp. AJ110404
2.1.2.7 column chromatography, 72.8% yield Aminobacter sp. AJ110403

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.2.7 additional information
-
 1.25 U/mg, cell-free extract Ensifer sp. AJ110404
2.1.2.7 additional information
-
 4.75 U/mg, cell-free extract Aminobacter sp. AJ110403
2.1.2.7 additional information
-
 7.10 U/mg, after purification Ensifer sp. AJ110404
2.1.2.7 additional information
-
 8.32 U/mg, after purification Aminobacter sp. AJ110403

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.7 2-methyl-L-serine + tetrahydrofolate assay at pH 7.4, 30°C Aminobacter sp. AJ110403 D-alanine + 5,10-methylenetetrahydrofolate
-
 r
2.1.2.7 2-methyl-L-serine + tetrahydrofolate assay at pH 7.4, 30°C Ensifer sp. AJ110404 D-alanine + 5,10-methylenetetrahydrofolate
-
 r

Subunits

EC Number Subunits Comment Organism
2.1.2.7 homodimer
-
 Aminobacter sp. AJ110403
2.1.2.7 homodimer
-
 Ensifer sp. AJ110404

Synonyms

EC Number Synonyms Comment Organism
2.1.2.7 alpha-methylserine hydroxymethyltransferase
-
 Aminobacter sp. AJ110403
2.1.2.7 alpha-methylserine hydroxymethyltransferase
-
 Ensifer sp. AJ110404
2.1.2.7 MSHMT
-
 Aminobacter sp. AJ110403
2.1.2.7 MSHMT
-
 Ensifer sp. AJ110404

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.2.7 30
-
 assay at Aminobacter sp. AJ110403
2.1.2.7 30
-
 assay at Ensifer sp. AJ110404

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.2.7 50
-
 value about Aminobacter sp. AJ110403
2.1.2.7 50
-
 value about Ensifer sp. AJ110404

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.2.7 7.4
-
 assay at Aminobacter sp. AJ110403
2.1.2.7 7.4
-
 assay at Ensifer sp. AJ110404

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.1.2.7 6 7
-
 Aminobacter sp. AJ110403
2.1.2.7 6 7.5
-
 Ensifer sp. AJ110404