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Literature summary extracted from

  • Kurisaki, A.; Sagami, H.; Ogura, K.
    Proteolytic release of dehydrodolichyl diphosphate synthase from pig testis microsomes (1996), Biosci. Biotechnol. Biochem., 60, 1109-1114.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.87 deoxycholate microsomal enzyme and enzyme released from microsomes are maximally activated at 5 mM. In the absence of deoxycholate, the released enzyme is inactive, while the microsomal enzyme has significant activity Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.87 KCl 0.4 M, more than 90% inhibition Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.87 0.000532
-
 isopentenyl diphosphate pH 8.5, 37°C, enzyme released from microsomes Sus scrofa
2.5.1.87 0.000538
-
 isopentenyl diphosphate pH 8.5, 37°C, microsomal enzyme Sus scrofa
2.5.1.87 0.013
-
 (2E,6E)-farnesyl diphosphate pH 8.5, 37°C, microsomal enzyme Sus scrofa
2.5.1.87 0.0149
-
 (2E,6E)-farnesyl diphosphate pH 8.5, 37°C, enzyme released from microsomes Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.87 microsome
-
 Sus scrofa
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.87 Mg2+ maximal activity at 1 mM Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.87 additional information
-
 gel filtration of the released enzyme gives a peak of dehydrodolichyl diphosphate synthase activity, which appears between 150000 Da and 50000 Da molecular mass markers Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.87 Sus scrofa
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.87 testis
-
 Sus scrofa
-

Storage Stability

EC Number Storage Stability Organism
2.5.1.87 -80°C, presence of glycerol is indispensable Sus scrofa
2.5.1.87 4°C, 5 days, 20% glycerol, more than 60% of enzyme activity is retained Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.87 (2E,6E)-farnesyl diphosphate + isopentenyl diphosphate
-
 Sus scrofa diphosphate + di-trans,poly-cis-polyprenyl diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.87 dehydrodolichyl diphosphate synthase
-
 Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.87 37
-
 assay at Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.87 8.5
-
-
 Sus scrofa